Calcium-Permeable AMPA Receptors Mediate the Induction of the Protein Kinase A-Dependent Component of Long-Term Potentiation in the Hippocampus

Pojeong Park, Tom Sanderson, Mascia Amici, Sun-Lim Choi, Zuner Bortolotto, M Zhuo, BK Kaang, Graham Collingridge

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Abstract

Two forms of NMDA receptor (NMDAR) dependent long-term potentiation (LTP) at hippocampal CA1 synapses can be distinguished based on their sensitivity to inhibitors of protein kinase A (PKA). The PKA-dependent form requires multiple episodes of high frequency stimulation (HFS) or theta burst stimuli (TBS) with a spacing between episodes in the order of minutes. To investigate the mechanism by which spaced episodes induce the PKA-dependent form of LTP we have compared, in interleaved experiments, spaced (s) and compressed (c) TBS protocols in the rat CA1 synapses. We find that LTP induced by sTBS, but not that induced by cTBS, involves the insertion of calcium-permeable (CP) AMPARs, as assessed using pharmacological and electrophysiological criteria. Furthermore, a single TBS when paired with rolipram, to activate PKA, generates an LTP that also involves the insertion of CP-AMPARs. These data demonstrate that the involvement of CP-AMPARs in LTP is critically determined by the timing of the induction trigger and is specifically associated with the PKA-dependent form of LTP.
Original languageEnglish
Pages (from-to)622-631
Number of pages10
JournalJournal of Neuroscience
Volume36
Issue number2
DOIs
Publication statusPublished - 13 Jan 2016

Keywords

  • calcium-permeable AMPA receptor
  • hippocampus
  • long-term potentiation
  • NMDA receptor
  • PKA
  • rectification

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