Calcium Sparks - Elementary Events Underlying Excitation-Contraction Coupling in Heart-Muscle

H Cheng, W J Lederer, M B Cannell

Research output: Contribution to journalArticle (Academic Journal)

1515 Citations (Scopus)

Abstract

Spontaneous local increases in the concentration of intracellular calcium, called ''calcium sparks,'' were detected in quiescent rat heart cells with a laser scanning confocal microscope and the fluorescent calcium indicator fluo-3. Estimates of calcium flux associated with the sparks suggest that calcium sparks result from spontaneous openings of single sarcoplasmic reticulum (SR) calcium-release channels, a finding supported by ryanodine-dependent changes of spark kinetics. At resting intracellular calcium concentrations, these SR calcium-release channels had a low rate of opening (approximately 0.0001 per second). An increase in the calcium content of the SR, however, was associated with a fourfold increase in opening rate and resulted in some sparks triggering propagating waves of increased intracellular calcium concentration. The calcium spark is the consequence of elementary events underlying excitation-contraction coupling and provides an explanation for both spontaneous and triggered changes in the intracellular calcium concentration in the mammalian heart.
Original languageEnglish
Pages (from-to)740-744
Number of pages5
JournalScience
Volume262
Issue number5134
Publication statusPublished - 29 Jan 1993

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