TY - JOUR
T1 - cAMP-dependent protein kinase A mediation of vasopressin gene expression in the hypothalamus of the osmotically challenged rat
AU - Wong, Liang Fong
AU - Harding, Tom
AU - Uney, James
AU - Murphy, David
PY - 2003/9/1
Y1 - 2003/9/1
N2 - We have tested the hypothesis that 3′, 5′-cyclic adenosine monophosphate (cAMP)-dependent protein kinase A (PKA) is involved in the regulation of the vasopressin (VP) gene in the magnocellular neurons of the paraventricular nucleus (PVN) of the osmotically challenged rat. An adenoviral vector expressing a potent peptide inhibitor of PKA, Ad.CMV.PKIα, was demonstrated to be highly efficient in vitro. Ad.CMV.PKIα was then introduced into the PVN of rats bearing a VP reporter transgene (3-VCAT-3) consisting of the VP structural gene containing an epitope reporter in exon III, flanked by 3 kb of upstream and 3 kb of downstream sequence Robust transgene expression is seen in VP neurons of the PVN, and this increases following 72 h of dehydration. Ad.CMV.PKIα significantly blunted 3-VCAT-3 expression in the osmotically stimulated PVN. Our evidence suggests that PKA mediates changes in VP gene expression in response to dehydration through sequences contained within the 3-VCAT-3 transgene.
AB - We have tested the hypothesis that 3′, 5′-cyclic adenosine monophosphate (cAMP)-dependent protein kinase A (PKA) is involved in the regulation of the vasopressin (VP) gene in the magnocellular neurons of the paraventricular nucleus (PVN) of the osmotically challenged rat. An adenoviral vector expressing a potent peptide inhibitor of PKA, Ad.CMV.PKIα, was demonstrated to be highly efficient in vitro. Ad.CMV.PKIα was then introduced into the PVN of rats bearing a VP reporter transgene (3-VCAT-3) consisting of the VP structural gene containing an epitope reporter in exon III, flanked by 3 kb of upstream and 3 kb of downstream sequence Robust transgene expression is seen in VP neurons of the PVN, and this increases following 72 h of dehydration. Ad.CMV.PKIα significantly blunted 3-VCAT-3 expression in the osmotically stimulated PVN. Our evidence suggests that PKA mediates changes in VP gene expression in response to dehydration through sequences contained within the 3-VCAT-3 transgene.
UR - http://www.scopus.com/inward/record.url?scp=0141503494&partnerID=8YFLogxK
U2 - 10.1016/S1044-7431(03)00123-4
DO - 10.1016/S1044-7431(03)00123-4
M3 - Article (Academic Journal)
C2 - 14550770
AN - SCOPUS:0141503494
SN - 1044-7431
VL - 24
SP - 82
EP - 90
JO - Molecular and Cellular Neuroscience
JF - Molecular and Cellular Neuroscience
IS - 1
ER -