Carbohydrate binding modules enhance cellulose enzymatic hydrolysis by increasing access of cellulases to the substrate

Amanda Bernardes, Vanessa Pellegrini, F Curtolo, César Camilo, Bruno Mello, Marcus Johns, Janet Scott, Francisco Guimarães, Igor Polikarpov

Research output: Contribution to journalArticle (Academic Journal)peer-review

19 Citations (Scopus)

Abstract

Plant biomass is a low-cost and abundant source of carbohydrates for production of fuels, “green” chemicals and materials. Currently, biochemical conversion of the biomass into sugars via enzymatic hydrolysis is the most viable technology. Here, the role of carbohydrate binding modules (CBMs) in the disruption of insoluble polysaccharide structures and their capacity to enhance cellulase-promoted lignocellulosic biomass hydrolysis was investigated. We show that CBM addition promotes generation of additional reducing ends in the insoluble substrate by cellulases. On the contrary, bovine serum albumin (BSA), widely used in prevention of a non-specific protein binding, causes an increase in soluble reducing-end production, when applied jointly with cellulases. We demonstrate that binding of CBMs to cellulose is non-homogeneous, irreversible and leads to its amorphisation. Our results also reveal effects of CBM-promoted amorphogenesis on cellulose hydrolysis by cellulases.
Original languageEnglish
Pages (from-to)57-68
Number of pages12
JournalCarbohydrate Polymers
Volume211
Early online date31 Jan 2019
DOIs
Publication statusPublished - 1 May 2019

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