Cardiac pericytes can be pharmacologically redirected towards a smooth muscle phenotype to enhance the revascularisation of the ischemic heart

Elisa Avolio*, Rajesh G Katare, Anita C Thomas, Andrea Caporali, Daryl Schwenke, Marco Meloni, Massimo Caputo, Paolo R Madeddu

*Corresponding author for this work

Research output: Contribution to conferenceConference Abstractpeer-review

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Abstract

Background: Pericytes (PC) are abundant cells that wrap around the whole vasculature of the heart. To date, it is not known if PC contribute to the maturation of new arterioles in the ischemic heart.
Purpose: we verified whether cardiac PC differentiate into contractile vascular smooth muscle cells (VSMC) in vitro, and if this potential can be pharmacologically induced to enhance the revascularisation of the heart post ischemia in vivo.
Methods and Results: PC were extracted from adult myocardial tissue, expanded in vitro, and characterised for antigenic profile and function. At baseline, PC do not express contractile VSMC antigens αSMA, CALP, SM22α, SM-MHC and Smoothelin B. We discovered that the inhibition of MEK1/2 activity and ERK1/2 signalling using the small molecule PD0325901 redirects the PC towards a VSMC phenotype. Contraction, calcium flux and migration assays confirmed that differentiated PC became stationary contractile cells, phenocopying control coronary artery SMC (CASMC). We further verified this finding using next-generation RNA-sequencing, which showed that differentiated PC expressed a cluster of contractile VSM transcripts similarly to control CASMC. Moreover, treated PC acquired a unique pro-angiogenic transcriptional profile, upregulating pro-angiogenic genes LEP and PDGFB while downregulating potent angiogenesis inhibitors ANGPT2, TIE1 and SERPINF1. We also verified that human PC acquire contractile VSM markers when injected subcutaneously in mice. We next validated this innovative approach in vivo. Healthy C57BL6/J mice given the drug orally, for two weeks, showed an increase in the small arteriole density along with an improved myocardial perfusion, when compared with mice given vehicle. Last, administration of PD0325901 to mice with myocardial infarction improved left ventricular function and induced an increase in both capillary and arteriole density in the peri-infarct area, when compared with controls given vehicle. Improved revascularisation resulted in reducing infarct expansion in PD0325901-treated mice.
Conclusion: we propose a novel therapeutic approach based on MEK inhibition to promote the revascularisation of the infarcted heart reawakening the plasticity of resident PC. This approach could benefit the treatment of patients with coronary artery disease.
Original languageEnglish
Pages13
Number of pages1
Publication statusUnpublished - 15 Feb 2021
EventESC Heart Failure Association Winter Meeting 2021 -
Duration: 20 Jan 202121 Jan 2021

Conference

ConferenceESC Heart Failure Association Winter Meeting 2021
Period20/01/2121/01/21

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