Cardioprotection by temperature preconditioning is mediated by free radicals, PKC and AMPK activation

I Khaliulin, M-S Suleiman, SJ Clarke, H Lin, J Parker, AP Halestrap

Research output: Contribution to journalArticle (Academic Journal)

Abstract

Isolated rat hearts were perfused for 40 min, followed by 25 min global ischaemia and 60 min reperfusion (37 °C). During preischaemia, hearts of ischemic preconditioning (IP) group underwent 3 cycles of 2 min global ischaemia and 3 min reperfusion at 37 °C whereas temperature preconditioning (TP) hearts received 3 cycles of 2 min hypothermic perfusion (26 °C) interspersed by 3 min normothermic perfusion (37 °C). Other hearts received a single 6 min hypothermic perfusion (SHP) before ischaemia. During reperfusion, TP improved hemodynamic recovery, decreased arrhythmias, reduced lactate dehydrogenase release and mitochondrial permeability transition pore opening more than IP or SHP. Both TP and IP were blocked by pretreatment with 10 μM chelerythrine (PKC inhibitor) and 300 μM mercapto-propionyl glycine (free radical scavenger). 10 μM Compound C (AMPK inhibitor) partially blocked cardioprotection by TP but not IP suggesting that free radicals, PKC and AMPK may mediate the effects of TP.
Translated title of the contributionCardioprotection by temperature preconditioning is mediated by free radicals, PKC and AMPK activation
Original languageEnglish
Pages (from-to)S177 - S178
Number of pages2
JournalJournal of Molecular and Cellular Cardiology
Volume42 (6, Suppl 1)
DOIs
Publication statusPublished - Jun 2007

Bibliographical note

Publisher: Elsevier

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