Cartilage tissue engineering by expanded goat articular chondrocytes

S Miot, P Scandiucci de Freitas, D Wirz, AU Daniels, TJ Sims, AP Hollander, P Mainil-Varlet, M Heberer, I Martin

Research output: Contribution to journalArticle (Academic Journal)peer-review

41 Citations (Scopus)

Abstract

In this study we investigated whether expanded goat chondrocytes have the capacity to generate cartilaginous tissues with biochemical and biomechanical properties improving with time in culture. Goat chondrocytes were expanded in monolayer with or without combinations of FGF-2, TGF-beta1, and PDGFbb, and the postexpansion chondrogenic capacity assessed in pellet cultures. Expanded chondrocytes were also cultured for up to 6 weeks in HYAFF(R)-M nonwoven meshes or Polyactivetrade mark foams, and the resulting cartilaginous tissues were assessed histologically, biochemically, and biomechanically. Supplementation of the expansion medium with FGF-2 increased the proliferation rate of goat chondrocytes and enhanced their postexpansion chondrogenic capacity. FGF-2-expanded chondrocytes seeded in HYAFF(R)-M or Polyactivetrade mark scaffolds formed cartilaginous tissues with wet weight, glycosaminoglycan, and collagen content, increasing from 2 days to 6 weeks culture (up to respectively 2-, 8-, and 41-fold). Equilibrium and dynamic stiffness measured in HYAFF(R)-M-based constructs also increased with time, up to, respectively, 1.3- and 16-fold. This study demonstrates the feasibility to engineer goat cartilaginous tissues at different stages of development by varying culture time, and thus opens the possibility to test the effect of maturation stage of engineered cartilage on the outcome of cartilage repair in orthotopic goat models.
Translated title of the contributionCartilage tissue engineering by expanded goat articular chondrocytes
Original languageEnglish
Pages (from-to)1078 - 1085
Number of pages8
JournalJournal of Orthopaedic Research
Volume24 (5)
DOIs
Publication statusPublished - May 2006

Bibliographical note

Publisher: Wiley

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