CD200R signaling inhibits pro-angiogenic gene expression by macrophages and suppresses choroidal neovascularization

Shintaro Horie, Scott J Robbie, Jian Liu, Wei-Kang Wu, Robin R Ali, James W Bainbridge, Lindsay B Nicholson, Manabu Mochizuki, Andrew D Dick, Dave A Copland

Research output: Contribution to journalArticle (Academic Journal)peer-review

27 Citations (Scopus)

Abstract

Macrophages are rapidly conditioned by cognate and soluble signals to acquire phenotypes that deliver specific functions during inflammation, wound healing and angiogenesis. Whether inhibitory CD200R signaling regulates pro-angiogenic macrophage phenotypes with the potential to suppress ocular neovascularization is unknown. CD200R-deficient bone marrow derived macrophages (BMMΦ) were used to demonstrate that macrophages lacking this inhibitory receptor exhibit enhanced levels of Vegfa, Arg-1 and Il-1β when stimulated with PGE2 or RPE-conditioned (PGE2-enriched) media. Endothelial tube formation in HUVECs was increased when co-cultured with PGE2-conditioned CD200R(-/-) BMMΦ, and laser-induced choroidal neovascularization was enhanced in CD200R-deficient mice. In corroboration, signaling through CD200R results in the down-regulation of BMMΦ angiogenic and pro-inflammatory phenotypes. Translational potential of this pathway was investigated in the laser-induced model of choroidal neovascularization. Local delivery of a CD200R agonist mAb to target myeloid infiltrate alters macrophage phenotype and inhibits pro-angiogenic gene expression, which suppresses pathological angiogenesis and CNV development.
Original languageEnglish
Pages (from-to)3072
JournalScientific Reports
Volume3
DOIs
Publication statusPublished - 2013

Fingerprint

Dive into the research topics of 'CD200R signaling inhibits pro-angiogenic gene expression by macrophages and suppresses choroidal neovascularization'. Together they form a unique fingerprint.

Cite this