Cell-free Synthesis of Macromolecular Complexes

Mathieu Botte, Aurelien Deniaud, Christiane Schaffitzel

Research output: Chapter in Book/Report/Conference proceedingChapter in a book

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Abstract

Cell-free protein synthesis based on E. coli cell extracts has been described for the first time more than 50 years ago. To date, cell-free synthesis is widely used for the preparation of toxic proteins, for studies of the translation process and its regulation as well as for the incorporation of artificial or labeled amino acids into a polypeptide chain. Many efforts have been directed towards establishing cell-free expression as a standard method for gene expression, with limited success. In this chapter we will describe the state-of-the-art of cell-free expression, extract preparation methods and recent examples for successful applications of cell-free synthesis of macromolecular complexes.
Original languageEnglish
Title of host publicationAdvanced Technologies for Protein Complex Production and Characterization
EditorsM. Cristina Vega
PublisherSpringer International Publishing AG
Pages79-95
Number of pages17
ISBN (Electronic)9783319272160
ISBN (Print)9783319272146
DOIs
Publication statusPublished - 11 May 2016

Publication series

NameAdvances in Experimental Medicine and Biology
PublisherSpringer International Publishing
Volume896
ISSN (Print)0065-2598

Structured keywords

  • Bristol BioDesign Institute

Keywords

  • Cell-free synthesis
  • Ribosomes
  • Membrane proteins
  • Synthetic biology

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