Cell-Specific Targeting of Genetically Encoded Tools for Neuroscience

Lucas Sjulson; Daniela Cassataro; Shamik DasGupta; Gero Miesenböck

doi:10.1146/annurev-genet-120215-035011

Cell-Specific Targeting of Genetically Encoded Tools for Neuroscience

Lucas Sjulson, Daniela Cassataro, Shamik DasGupta, Gero Miesenböck

School of Physiology, Pharmacology & Neuroscience

Research output: Contribution to journal › Review article (Academic Journal) › peer-review

38 Citations (Scopus)

Abstract

Genetically encoded tools for visualizing and manipulating neurons in vivo have led to significant advances in neuroscience, in large part because of the ability to target expression to specific cell populations of interest. Current methods enable targeting based on marker gene expression, development, anatomical projection pattern, synaptic connectivity, and recent activity as well as combinations of these factors. Here, we review these methods, focusing on issues of practical implementation as well as areas for future improvement.

Original language	English
Pages (from-to)	571-594
Number of pages	24
Journal	Annual Review of Genetics
Volume	50
DOIs	https://doi.org/10.1146/annurev-genet-120215-035011
Publication status	Published - 23 Nov 2016

Keywords

Animals
Animals, Genetically Modified
Gene Expression
Gene Transfer Techniques
Genetic Techniques
Humans
Neurons/physiology
Neurosciences/methods
Promoter Regions, Genetic
Transgenes

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10.1146/annurev-genet-120215-035011

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Dr Shamik Dasgupta
- shamik.dasguptabristol.acuk
- School of Physiology, Pharmacology & Neuroscience - Senior Research Fellow
Person: Academic

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title = "Cell-Specific Targeting of Genetically Encoded Tools for Neuroscience",

abstract = "Genetically encoded tools for visualizing and manipulating neurons in vivo have led to significant advances in neuroscience, in large part because of the ability to target expression to specific cell populations of interest. Current methods enable targeting based on marker gene expression, development, anatomical projection pattern, synaptic connectivity, and recent activity as well as combinations of these factors. Here, we review these methods, focusing on issues of practical implementation as well as areas for future improvement.",

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author = "Lucas Sjulson and Daniela Cassataro and Shamik DasGupta and Gero Miesenb{\"o}ck",

year = "2016",

month = nov,

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doi = "10.1146/annurev-genet-120215-035011",

language = "English",

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TY - JOUR

T1 - Cell-Specific Targeting of Genetically Encoded Tools for Neuroscience

AU - Sjulson, Lucas

AU - Cassataro, Daniela

AU - DasGupta, Shamik

AU - Miesenböck, Gero

PY - 2016/11/23

Y1 - 2016/11/23

N2 - Genetically encoded tools for visualizing and manipulating neurons in vivo have led to significant advances in neuroscience, in large part because of the ability to target expression to specific cell populations of interest. Current methods enable targeting based on marker gene expression, development, anatomical projection pattern, synaptic connectivity, and recent activity as well as combinations of these factors. Here, we review these methods, focusing on issues of practical implementation as well as areas for future improvement.

AB - Genetically encoded tools for visualizing and manipulating neurons in vivo have led to significant advances in neuroscience, in large part because of the ability to target expression to specific cell populations of interest. Current methods enable targeting based on marker gene expression, development, anatomical projection pattern, synaptic connectivity, and recent activity as well as combinations of these factors. Here, we review these methods, focusing on issues of practical implementation as well as areas for future improvement.

KW - Animals

KW - Animals, Genetically Modified

KW - Gene Expression

KW - Gene Transfer Techniques

KW - Genetic Techniques

KW - Humans

KW - Neurons/physiology

KW - Neurosciences/methods

KW - Promoter Regions, Genetic

KW - Transgenes

U2 - 10.1146/annurev-genet-120215-035011

DO - 10.1146/annurev-genet-120215-035011

M3 - Review article (Academic Journal)

C2 - 27732792

SN - 0066-4197

VL - 50

SP - 571

EP - 594

JO - Annual Review of Genetics

JF - Annual Review of Genetics

ER -

Cell-Specific Targeting of Genetically Encoded Tools for Neuroscience

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Dr Shamik Dasgupta

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