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Cell-based enzyme-linked immunosorbent assay (cell-ELISA) analysis of native and recombinant glutamate receptors

Research output: Chapter in Book/Report/Conference proceedingChapter in a book

Standard

Cell-based enzyme-linked immunosorbent assay (cell-ELISA) analysis of native and recombinant glutamate receptors. / Molnár, Elek.

Methods in Molecular Biology: Glutamate receptors: Methods and protocols. Springer, 2019. p. 47-54 (Methods in Molecular Biology; Vol. 1941).

Research output: Chapter in Book/Report/Conference proceedingChapter in a book

Harvard

Molnár, E 2019, Cell-based enzyme-linked immunosorbent assay (cell-ELISA) analysis of native and recombinant glutamate receptors. in Methods in Molecular Biology: Glutamate receptors: Methods and protocols. Methods in Molecular Biology, vol. 1941, Springer, pp. 47-54. https://doi.org/10.1007/978-1-4939-9077-1_4, https://doi.org/10.1007/978-1-4939-9077-1_4

APA

Molnár, E. (2019). Cell-based enzyme-linked immunosorbent assay (cell-ELISA) analysis of native and recombinant glutamate receptors. In Methods in Molecular Biology: Glutamate receptors: Methods and protocols (pp. 47-54). (Methods in Molecular Biology; Vol. 1941). Springer. https://doi.org/10.1007/978-1-4939-9077-1_4, https://doi.org/10.1007/978-1-4939-9077-1_4

Vancouver

Molnár E. Cell-based enzyme-linked immunosorbent assay (cell-ELISA) analysis of native and recombinant glutamate receptors. In Methods in Molecular Biology: Glutamate receptors: Methods and protocols. Springer. 2019. p. 47-54. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-9077-1_4, https://doi.org/10.1007/978-1-4939-9077-1_4

Author

Molnár, Elek. / Cell-based enzyme-linked immunosorbent assay (cell-ELISA) analysis of native and recombinant glutamate receptors. Methods in Molecular Biology: Glutamate receptors: Methods and protocols. Springer, 2019. pp. 47-54 (Methods in Molecular Biology).

Bibtex

@inbook{2b787f068418400faca8e526e7dea01c,
title = "Cell-based enzyme-linked immunosorbent assay (cell-ELISA) analysis of native and recombinant glutamate receptors",
abstract = "Glutamate receptors (GluRs) located primarily on the membranes of neurons and glial cells are responsible for excitatory synaptic transmission in the central nervous system. The transport of GluRs to the cell surface is a highly regulated dynamic process that determines neuronal excitability and synaptic responses. The molecular and cellular mechanisms of GluR trafficking are often studied in cell cultures. These studies require sensitive techniques that allow the measurement of total and surface expressed GluRs in cell populations. The cell-based enzyme-linked immunosorbent assay (cell-ELISA) combines steps of direct immunochemical labelling of cell cultures and ELISA. It can be used for quantitative comparisons of surface expressed and total protein contents of various cell cultures. While several cell-ELISA protocols are available for different cell types, in this chapter we describe the procedure that we have applied for the investigation of quantitative changes in the cell surface expression of recombinant ionotropic glutamate receptors (iGluRs) in adherent human embryonic kidney 293 (HEK293) cells and endogenous iGluR proteins in primary neuronal cultures.",
keywords = "Antibodies, Cell-ELISA, Cell surface protein, Glutamate receptor, Neuronal culture, Targeting, trafficking, Neurochemistry, Receptors, Membrane proteins, Cell biology, Cell culture",
author = "Elek Moln{\'a}r",
year = "2019",
month = "3",
day = "23",
doi = "10.1007/978-1-4939-9077-1_4",
language = "English",
isbn = "978-1-4939-9077-1",
series = "Methods in Molecular Biology",
publisher = "Springer",
pages = "47--54",
booktitle = "Methods in Molecular Biology",

}

RIS - suitable for import to EndNote

TY - CHAP

T1 - Cell-based enzyme-linked immunosorbent assay (cell-ELISA) analysis of native and recombinant glutamate receptors

AU - Molnár, Elek

PY - 2019/3/23

Y1 - 2019/3/23

N2 - Glutamate receptors (GluRs) located primarily on the membranes of neurons and glial cells are responsible for excitatory synaptic transmission in the central nervous system. The transport of GluRs to the cell surface is a highly regulated dynamic process that determines neuronal excitability and synaptic responses. The molecular and cellular mechanisms of GluR trafficking are often studied in cell cultures. These studies require sensitive techniques that allow the measurement of total and surface expressed GluRs in cell populations. The cell-based enzyme-linked immunosorbent assay (cell-ELISA) combines steps of direct immunochemical labelling of cell cultures and ELISA. It can be used for quantitative comparisons of surface expressed and total protein contents of various cell cultures. While several cell-ELISA protocols are available for different cell types, in this chapter we describe the procedure that we have applied for the investigation of quantitative changes in the cell surface expression of recombinant ionotropic glutamate receptors (iGluRs) in adherent human embryonic kidney 293 (HEK293) cells and endogenous iGluR proteins in primary neuronal cultures.

AB - Glutamate receptors (GluRs) located primarily on the membranes of neurons and glial cells are responsible for excitatory synaptic transmission in the central nervous system. The transport of GluRs to the cell surface is a highly regulated dynamic process that determines neuronal excitability and synaptic responses. The molecular and cellular mechanisms of GluR trafficking are often studied in cell cultures. These studies require sensitive techniques that allow the measurement of total and surface expressed GluRs in cell populations. The cell-based enzyme-linked immunosorbent assay (cell-ELISA) combines steps of direct immunochemical labelling of cell cultures and ELISA. It can be used for quantitative comparisons of surface expressed and total protein contents of various cell cultures. While several cell-ELISA protocols are available for different cell types, in this chapter we describe the procedure that we have applied for the investigation of quantitative changes in the cell surface expression of recombinant ionotropic glutamate receptors (iGluRs) in adherent human embryonic kidney 293 (HEK293) cells and endogenous iGluR proteins in primary neuronal cultures.

KW - Antibodies

KW - Cell-ELISA

KW - Cell surface protein

KW - Glutamate receptor

KW - Neuronal culture

KW - Targeting

KW - trafficking

KW - Neurochemistry

KW - Receptors

KW - Membrane proteins

KW - Cell biology

KW - Cell culture

UR - http://www.scopus.com/inward/record.url?scp=85060921086&partnerID=8YFLogxK

U2 - 10.1007/978-1-4939-9077-1_4

DO - 10.1007/978-1-4939-9077-1_4

M3 - Chapter in a book

SN - 978-1-4939-9077-1

T3 - Methods in Molecular Biology

SP - 47

EP - 54

BT - Methods in Molecular Biology

PB - Springer

ER -