Chapter six - Measuring membrane protein dynamics in neurons using fluorescence recovery after photobleach

IM González-González; F Jaskolski; Y Goldberg; MC Ashby; JM Henley

doi:10.1016/B978-0-12-391857-4.00006-9

Chapter six - Measuring membrane protein dynamics in neurons using fluorescence recovery after photobleach

IM González-González, F Jaskolski, Y Goldberg, MC Ashby, JM Henley

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Abstract

The use of genetically encoded fluorescent tags such as green fluorescent protein (GFP) as reporters to monitor processes in living cells has transformed cell biology. One major application for these tools has been to analyze protein dynamics in neurons. In particular, fluorescence recovery after photobleach (FRAP) of surface expressed fluorophore-tagged proteins has been instrumental to addressing outstanding questions about how neurons orchestrate the synaptic delivery of proteins. Here, we provide an overview of the methodology, equipment, and analysis required to perform, analyze, and interpret these experiments.

Translated title of the contribution	Chapter six - Measuring membrane protein dynamics in neurons using fluorescence recovery after photobleach
Original language	English
Pages (from-to)	127 - 146
Number of pages	20
Journal	Methods in Enzymology
Volume	504
DOIs	https://doi.org/10.1016/B978-0-12-391857-4.00006-9
Publication status	Published - 2012

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Henley, J. M. (Principal Investigator)
1/01/08 → 1/01/13
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abstract = "The use of genetically encoded fluorescent tags such as green fluorescent protein (GFP) as reporters to monitor processes in living cells has transformed cell biology. One major application for these tools has been to analyze protein dynamics in neurons. In particular, fluorescence recovery after photobleach (FRAP) of surface expressed fluorophore-tagged proteins has been instrumental to addressing outstanding questions about how neurons orchestrate the synaptic delivery of proteins. Here, we provide an overview of the methodology, equipment, and analysis required to perform, analyze, and interpret these experiments.",

author = "IM Gonz{\'a}lez-Gonz{\'a}lez and F Jaskolski and Y Goldberg and MC Ashby and JM Henley",

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AU - González-González, IM

AU - Jaskolski, F

AU - Goldberg, Y

AU - Ashby, MC

AU - Henley, JM

PY - 2012

Y1 - 2012

N2 - The use of genetically encoded fluorescent tags such as green fluorescent protein (GFP) as reporters to monitor processes in living cells has transformed cell biology. One major application for these tools has been to analyze protein dynamics in neurons. In particular, fluorescence recovery after photobleach (FRAP) of surface expressed fluorophore-tagged proteins has been instrumental to addressing outstanding questions about how neurons orchestrate the synaptic delivery of proteins. Here, we provide an overview of the methodology, equipment, and analysis required to perform, analyze, and interpret these experiments.

AB - The use of genetically encoded fluorescent tags such as green fluorescent protein (GFP) as reporters to monitor processes in living cells has transformed cell biology. One major application for these tools has been to analyze protein dynamics in neurons. In particular, fluorescence recovery after photobleach (FRAP) of surface expressed fluorophore-tagged proteins has been instrumental to addressing outstanding questions about how neurons orchestrate the synaptic delivery of proteins. Here, we provide an overview of the methodology, equipment, and analysis required to perform, analyze, and interpret these experiments.

U2 - 10.1016/B978-0-12-391857-4.00006-9

DO - 10.1016/B978-0-12-391857-4.00006-9

M3 - Article (Academic Journal)

C2 - 22264532

SN - 1557-7988

VL - 504

SP - 127

EP - 146

JO - Methods in Enzymology

JF - Methods in Enzymology

ER -

Chapter six - Measuring membrane protein dynamics in neurons using fluorescence recovery after photobleach

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