Characterisation of Flavonoid Aglycones by Negative Ion Chip-Based Nanospray Tandem Mass Spectrometry

Paul J. Gates*, Norberto P. Lopes

*Corresponding author for this work

Research output: Contribution to journalArticle (Academic Journal)peer-review

Abstract

Flavonoids are one of the most important classes of natural products having a wide variety of biological activities. There is wide interest in a range of medical and dietary applications, and having a rapid, reliable method for structural elucidation is essential. In this study a range of flavonoid standards are investigated by chip-based negative ion nanospray mass spectrometry. It was found that the different classes of flavonoid studied have a combination of distinct neutral losses from the precursor ion [M-H](-) along with characteristic low-mass ions. By looking only for this distinct pattern of product ions, it is possible to determine the class of flavonoid directly. This methodology is tested here by the analysis of a green tea extract, where the expected flavonoids were readily identified, along with quercetin, which is shown to be present at only about 2% of the most intense ion in the spectrum.

Translated title of the contributionCharacterisation of Flavonoid Aglycones by Negative Ion Chip-Based Nanospray Tandem Mass Spectrometry
Original languageEnglish
Article number259217
Pages (from-to)1 - 7
Number of pages7
JournalInternational Journal of Analytical Chemistry
Volume2012
DOIs
Publication statusPublished - Feb 2012

Bibliographical note

Publisher: Hindawi Publishing Corporation

Keywords

  • ELECTROSPRAY-IONIZATION
  • VIROLA-SURINAMENSIS
  • MOLECULAR-IONS
  • ASTERACEAE
  • FRAGMENTATION
  • ANTHOCYANINS
  • CONSTITUENTS
  • POLYENES

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