Characterisation of the IGF axis & Wilms' Tumour 1 tumour suppressor gene in hyperparathyroidism

CKM Wong, T Lai, SJ White, E Sheffield, MH Wheeler, C Stewart, JR Farndon

Research output: Contribution to journalArticle (Academic Journal)

4 Citations (Scopus)

Abstract

Abstract: Background: Genetic mutations and upregulation of growth factors are implicated in the pathogenesis of hyperparathyroidism. The aim of this study was to evaluate the role of Wilms' tumour suppressor gene (WT-1) and the insulin-like growth factor (IGF) axis in hyperparathyroidism. Methods: The expression of WT-1 and IGF components was examined by immunohistochemistry, reverse transcriptase-polymerase chain reaction and western immunoblotting in a panel of parathyroid specimens from both primary and secondary hyperparathyroidism. A human parathyroid cell culture model was established to examine the parathyroid response to IGF stimulation. Results: There was a significantly lower level of WT-1 expression in parathyroid tumours than in normal parathyroid glands. Most tumours expressed IGF-I and IGF-II receptors and responded to IGF stimulation. Only IGF-I was present in normal parathyroid glands, whereas IGF-II was expressed exclusively in parathyroid tumours. Conclusion: Abnormal expression of WT-1 and the IGF axis may play a role in the pathogenesis of hyperparathyroidism.
Translated title of the contributionCharacterisation of the IGF axis & Wilms' Tumour 1 tumour suppressor gene in hyperparathyroidism
Original languageEnglish
Pages (from-to)1232 - 1241
Number of pages10
JournalBritish Journal of Surgery
Volume94
DOIs
Publication statusPublished - 2007

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    Wong, CKM., Lai, T., White, SJ., Sheffield, E., Wheeler, MH., Stewart, C., & Farndon, JR. (2007). Characterisation of the IGF axis & Wilms' Tumour 1 tumour suppressor gene in hyperparathyroidism. British Journal of Surgery, 94, 1232 - 1241. https://doi.org/10.1002/bjs.5566