Characterization and differential nuclear localization of Nopp140 and a novel Nopp140-like protein in trypanosomes

S Kelly, Will G B Singleton, B Wickstead, K Ersfeld, K Gull

Research output: Contribution to journalArticle (Academic Journal)peer-review

Abstract

Trypanosomatids possess two homologues of Nopp140: a canonical Nopp140 and a Nopp140-like protein (TbNoLP) in which a GAR domain replaces the C-terminal SRP40 domain. Both are phosphorylated and coimmunoprecipitate with RNA polymerase I. Each paralogue has a distinct subnuclear localization, and depletion of TbNoLP produces an enlarged nucleolus in which TbNopp140-containing regions disperse. The restricted occurrence pattern of NoLP proteins reflects an intriguing convergence in evolution, suggestive of a function in nucleoplasmic small nucleolar ribonucleoprotein shuttling.

Original languageEnglish
Pages (from-to)876-9
Number of pages4
JournalEukaryotic Cell
Volume5
Issue number5
DOIs
Publication statusPublished - May 2006

Keywords

  • Amino Acid Sequence
  • Animals
  • Cell Nucleolus
  • Cell Nucleus
  • Molecular Sequence Data
  • Nuclear Proteins
  • Phosphoproteins
  • Phosphorylation
  • Protozoan Proteins
  • RNA Interference
  • RNA Polymerase I
  • Ribonucleoproteins, Small Nucleolar
  • Trypanosoma brucei brucei

Fingerprint Dive into the research topics of 'Characterization and differential nuclear localization of Nopp140 and a novel Nopp140-like protein in trypanosomes'. Together they form a unique fingerprint.

Cite this