Characterization and differential nuclear localization of Nopp140 and a novel Nopp140-like protein in trypanosomes

S Kelly; Will G B Singleton; B Wickstead; K Ersfeld; K Gull

doi:10.1128/EC.5.5.876-879.2006

Characterization and differential nuclear localization of Nopp140 and a novel Nopp140-like protein in trypanosomes

S Kelly, Will G B Singleton, B Wickstead, K Ersfeld, K Gull

Bristol Medical School (THS)

Research output: Contribution to journal › Article (Academic Journal) › peer-review

15 Citations (Scopus)

Abstract

Trypanosomatids possess two homologues of Nopp140: a canonical Nopp140 and a Nopp140-like protein (TbNoLP) in which a GAR domain replaces the C-terminal SRP40 domain. Both are phosphorylated and coimmunoprecipitate with RNA polymerase I. Each paralogue has a distinct subnuclear localization, and depletion of TbNoLP produces an enlarged nucleolus in which TbNopp140-containing regions disperse. The restricted occurrence pattern of NoLP proteins reflects an intriguing convergence in evolution, suggestive of a function in nucleoplasmic small nucleolar ribonucleoprotein shuttling.

Original language	English
Pages (from-to)	876-9
Number of pages	4
Journal	Eukaryotic Cell
Volume	5
Issue number	5
DOIs	https://doi.org/10.1128/EC.5.5.876-879.2006
Publication status	Published - May 2006

Keywords

Amino Acid Sequence
Animals
Cell Nucleolus
Cell Nucleus
Molecular Sequence Data
Nuclear Proteins
Phosphoproteins
Phosphorylation
Protozoan Proteins
RNA Interference
RNA Polymerase I
Ribonucleoproteins, Small Nucleolar
Trypanosoma brucei brucei

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title = "Characterization and differential nuclear localization of Nopp140 and a novel Nopp140-like protein in trypanosomes",

abstract = "Trypanosomatids possess two homologues of Nopp140: a canonical Nopp140 and a Nopp140-like protein (TbNoLP) in which a GAR domain replaces the C-terminal SRP40 domain. Both are phosphorylated and coimmunoprecipitate with RNA polymerase I. Each paralogue has a distinct subnuclear localization, and depletion of TbNoLP produces an enlarged nucleolus in which TbNopp140-containing regions disperse. The restricted occurrence pattern of NoLP proteins reflects an intriguing convergence in evolution, suggestive of a function in nucleoplasmic small nucleolar ribonucleoprotein shuttling.",

keywords = "Amino Acid Sequence, Animals, Cell Nucleolus, Cell Nucleus, Molecular Sequence Data, Nuclear Proteins, Phosphoproteins, Phosphorylation, Protozoan Proteins, RNA Interference, RNA Polymerase I, Ribonucleoproteins, Small Nucleolar, Trypanosoma brucei brucei",

author = "S Kelly and Singleton, {Will G B} and B Wickstead and K Ersfeld and K Gull",

year = "2006",

month = may,

doi = "10.1128/EC.5.5.876-879.2006",

language = "English",

volume = "5",

pages = "876--9",

journal = "Eukaryotic Cell",

issn = "1535-9778",

publisher = "American Society for Microbiology",

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TY - JOUR

T1 - Characterization and differential nuclear localization of Nopp140 and a novel Nopp140-like protein in trypanosomes

AU - Kelly, S

AU - Singleton, Will G B

AU - Wickstead, B

AU - Ersfeld, K

AU - Gull, K

PY - 2006/5

Y1 - 2006/5

N2 - Trypanosomatids possess two homologues of Nopp140: a canonical Nopp140 and a Nopp140-like protein (TbNoLP) in which a GAR domain replaces the C-terminal SRP40 domain. Both are phosphorylated and coimmunoprecipitate with RNA polymerase I. Each paralogue has a distinct subnuclear localization, and depletion of TbNoLP produces an enlarged nucleolus in which TbNopp140-containing regions disperse. The restricted occurrence pattern of NoLP proteins reflects an intriguing convergence in evolution, suggestive of a function in nucleoplasmic small nucleolar ribonucleoprotein shuttling.

AB - Trypanosomatids possess two homologues of Nopp140: a canonical Nopp140 and a Nopp140-like protein (TbNoLP) in which a GAR domain replaces the C-terminal SRP40 domain. Both are phosphorylated and coimmunoprecipitate with RNA polymerase I. Each paralogue has a distinct subnuclear localization, and depletion of TbNoLP produces an enlarged nucleolus in which TbNopp140-containing regions disperse. The restricted occurrence pattern of NoLP proteins reflects an intriguing convergence in evolution, suggestive of a function in nucleoplasmic small nucleolar ribonucleoprotein shuttling.

KW - Amino Acid Sequence

KW - Animals

KW - Cell Nucleolus

KW - Cell Nucleus

KW - Molecular Sequence Data

KW - Nuclear Proteins

KW - Phosphoproteins

KW - Phosphorylation

KW - Protozoan Proteins

KW - RNA Interference

KW - RNA Polymerase I

KW - Ribonucleoproteins, Small Nucleolar

KW - Trypanosoma brucei brucei

U2 - 10.1128/EC.5.5.876-879.2006

DO - 10.1128/EC.5.5.876-879.2006

M3 - Article (Academic Journal)

C2 - 16682465

SN - 1535-9778

VL - 5

SP - 876

EP - 879

JO - Eukaryotic Cell

JF - Eukaryotic Cell

IS - 5

ER -

Characterization and differential nuclear localization of Nopp140 and a novel Nopp140-like protein in trypanosomes

Abstract

Keywords

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