Characterization of multiple platelet activation pathways in patients with bleeding as a high-throughput screening option: use of 96-well Optimul assay

Marie Lordkipanidzé, Gillian C Lowe, Nicholas S Kirkby, Melissa V Chan, Martina H Lundberg, Neil V Morgan, Danai Bem, Shaista P Nisar, Vincenzo C Leo, Matthew L Jones, Stuart J Mundell, Martina E Daly, Andrew D Mumford, Timothy D Warner, Steve P Watson

Research output: Contribution to journalArticle (Academic Journal)peer-review

43 Citations (Scopus)

Abstract

Up to 1% of the general population have mild bleeding disorders, but these are often poorly characterized, particularly with regard to the roles of platelets. Here we have compared the usefulness of Optimul, a 96-well-plate-based assay of seven distinct pathways of platelet activation, to characterize inherited platelet defects in comparison to light transmission aggregometry (LTA). Using Optimul and LTA, concentration-response curves were generated for arachidonic acid, ADP, collagen, epinephrine, TRAP-6, U46619, and ristocetin in samples from (i) healthy volunteers (n=50), (ii) healthy volunteers treated with antiplatelet agents in vitro (n=10), and (iii) patients with bleeding of unknown origin (n=65). The assays gave concordant results in 82% of cases (κ=0.62, p
Original languageEnglish
Pages (from-to)e11-e22
Number of pages12
JournalBlood
Volume123
Issue number8
Early online date9 Jan 2014
DOIs
Publication statusPublished - 20 Feb 2014

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