Characterization of the polarized endothelial secretom

Haoche Wei, Ananthalakshmy Sundararaman, Emily J Dickson, Lewis Rennie-Campbell, Eloise Cross, Kate J Heesom, Harry Mellor

Research output: Contribution to journalArticle (Academic Journal)peer-review

19 Citations (Scopus)
302 Downloads (Pure)


Endothelial cells (ECs) form an active barrier between the circulation and the body. In addition to controlling transport of molecules between these 2 compartments, the endothelium is a major secretory organ, releasing proteins both into the circulation and into the vascular matrix. Although it is clearly important that proteins are correctly sorted into these 2 spaces, we currently know little of the polarization of this secretion or how it is controlled. Here, we present an optimized system for the analysis of polarized secretion and show that it allows the derivation of deep, robust proteomes from small numbers of primary ECs. We present the first endothelial apically and basolaterally secreted proteomes, demonstrating that ECs polarize the secretion of extracellular vesicle cargoes to the apical surface. Conversely, we find that protein secretion at the basolateral surface is focused on components of the extracellular matrix (ECM). Finally, we examine the role of liprin-α1 in secretion toward the basolateral compartment and identify a subset of ECM components that share this route with fibronectin.—Wei, H., Sundararaman, A., Dickson, E., Rennie-Campbell, L., Cross, E., Heesom, K. J., Mellor, H. Characterization of the polarized endothelial secretome.
Original languageEnglish
Number of pages11
JournalFASEB Journal
Early online date20 Aug 2019
Publication statusE-pub ahead of print - 20 Aug 2019


  • Endothelial cell
  • secretion
  • extracellular matrix
  • polarization
  • intracellular traffic


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