Chemogenetic activation of endogenous arginine vasopressin exerts anorexigenic effects via central nesfatin-1/NucB2 pathway

Kenya Sanada, Mitsuhiro Yoshimura, Naofumi Ikeda, Kazuhiko Baba, Haruki Nishimura, Kazuaki Nishimura, Yuki Nonaka, Takashi Maruyama, Tetsu Miyamoto, Masatomo Mori, Becky Conway-Campbell, Stafford Lightman, Masaharu Kataoka, Yoichi Ueta

Research output: Contribution to journalArticle (Academic Journal)peer-review

2 Downloads (Pure)

Abstract

We examined whether the chemogenetic activation of endogenous arginine vasopressin (AVP) affects central nesfatin-1/NucB2 neurons, using a transgenic rat line that was previously generated. Saline (1 mL/kg) or clozapine-N-oxide (CNO, 1 mg/mL/kg), an agonist for hM3Dq, was subcutaneously administered in adult male AVP-hM3Dq-mCherry transgenic rats (300-370 g). Food and water intake were significantly suppressed after subcutaneous (s.c.) injection of CNO, with aberrant circadian rhythmicity. The percentages of Fos expression in nesfatin-1/NucB2-immunoreactive neurons were significantly increased in the hypothalamus and brainstem at 120 min after s.c. injection of CNO. Suppressed food intake that was induced by chemogenetic activation of endogenous AVP was ablated after intracerebroventricularly administered nesfatin-1/NucB2-neutralizing antibody in comparison with vehicle, without any alteration of water intake nor circadian rhythmicity. These results suggest that chemogenetic activation of endogenous AVP affects, at least in part, central nesfatin-1/NucB2 neurons and may exert anorexigenic effects in the transgenic rats.

Original languageEnglish
Article number18
Pages (from-to)18
JournalThe journal of physiological sciences : JPS
Volume71
Issue number1
DOIs
Publication statusPublished - 16 Jun 2021

Bibliographical note

Funding Information:
This paper was supported by a Grant-in-Aid for Scientific Research (C) (21K06779) for MY from the Ministry of Education, Culture, Sports, Science, and Technology (MEXT), Japan and an UOEH Research Grant for Young Scientist for MY from the UOEH, Japan.

Publisher Copyright:
© 2021, The Author(s).

Fingerprint

Dive into the research topics of 'Chemogenetic activation of endogenous arginine vasopressin exerts anorexigenic effects via central nesfatin-1/NucB2 pathway'. Together they form a unique fingerprint.

Cite this