Chemoselective N-H insertion catalysed by a de novo carbene transferase.

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The ability to perform organic reactions with chemoselectivity is of critical importance in synthesis. Recently we reported that a de novo carbene transferase, a tetra‐α‐helical c‐type heme‐containing protein, C45, is proficient at N‐H insertion reactions, proceeding via the intermolecular transfer of a metallocarbenoid intermediate into the N‐H σ‐bond to form a new N‐C σ‐bond. Here we demonstrate that C45 can also catalyse N‐H insertion reactions chemoselectively, even when the substrate contains an unprotected hydroxyl group.
Original languageEnglish
JournalBiotechnology and Applied Biochemistry
Publication statusPublished - 22 May 2020


  • Biocatalysis
  • Carbene transferase
  • De novo protein design


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