Chronic hypoxia inhibits postnatal maturation of porcine intrapulmonary artery relaxation

R. M.R. Tulloh, A. A. Hislop, P. J. Boels, J. Deutsch

Research output: Contribution to journalArticle (Academic Journal)

68 Citations (Scopus)

Abstract

Neonatal pulmonary hypertension is associated with increased pulmonary vascular reactivity. We studied the responses of isolated porcine intrapulmonary arteries after exposure of piglets to chronic hypobaric hypoxia (CHH) from 0 to 2.5, 3 to 6, or 14 to 17 days of age. CHH inhibited the postnatal development of endothelium-dependent vasorelaxation to acetylcholine (ACh) and the calcium ionophore A-23187. Basal accumulation of guanosine 3', 5- -cyclic monophosphate (cGMP) was unaffected, but cGMP response to ACh was inhibited. Endothelium-independent relaxation to nitric oxide (NO) and zaprinast (a phosphodiesterase inhibitor) was also inhibited, but cGMP accumulation in response to these agonists was normal. The ability of sodium nitroprusside (SNP) to cause vasorelaxation and increase cGMP accumulation was unaffected. Contractile responses to potassium chloride and prostaglandin F(2α), (PGF(2α)) were similar to normal after exposure from birth and 3 days and were decreased in the older group, but the ability of N(G)-monomethyl-L-arginine acetate to increase PGF(2α)-induced contractions decreased. Thus exposure of newborn piglets to CHH causes 1) no increase in contractile responses and 2) impairment of endothelium-dependent and - independent relaxation by impairing signal transduction mechanisms involved in the release of NO and the effectiveness of cGMP.

Original languageEnglish
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume272
Issue number5 41-5
Publication statusPublished - 17 Jun 1997

Keywords

  • acetylcholine
  • endothelium
  • guanosine 3',5'-cyclic monophosphate
  • pulmonary hypertension
  • smooth muscle

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