Clasp-mediated microtubule bundling regulates persistent motility and contact repulsion in Drosophila macrophages in vivo

Brian Stramer, Severina Moreira, Tom Millard, Iwan Evans, Chieh-Yin Huang, Ola Sabet, Martin Milner, Graham Dunn, Paul Martin, Will Wood

Research output: Contribution to journalArticle (Academic Journal)peer-review

86 Citations (Scopus)

Abstract

Drosophila melanogaster macrophages are highly migratory cells that lend themselves beautifully to high resolution in vivo imaging experiments. By expressing fluorescent probes to reveal actin and microtubules, we can observe the dynamic interplay of these two cytoskeletal networks as macrophages migrate and interact with one another within a living organism. We show that before an episode of persistent motility, whether responding to developmental guidance or wound cues, macrophages assemble a polarized array of microtubules that bundle into a compass-like arm that appears to anticipate the direction of migration. Whenever cells collide with one another, their microtubule arms transiently align just before cell–cell repulsion, and we show that forcing depolymerization of microtubules by expression of Spastin leads to their defective polarity and failure to contact inhibit from one another. The same is true in orbit/clasp mutants, indicating a pivotal role for this microtubule-binding protein in the assembly and/or functioning of the microtubule arm during polarized migration and contact repulsion.
Translated title of the contributionClasp-mediated microtubule bundling regulates persistent motility and contact repulsion in Drosophila macrophages in vivo
Original languageEnglish
Article numberS1 - S4
Pages (from-to)681 - 689
Number of pages9
JournalJournal of Cell Biology
Volume189
Issue number4
DOIs
Publication statusPublished - 17 May 2010

Bibliographical note

Other: Also supplied image for front cover. Cover article plus featured BioByte and editorial choice in Science Vol 328, 18th June 2010

Keywords

  • Actin Cytoskeleton
  • Adenosine Triphosphatases
  • Animals
  • Drosophila Proteins
  • Drosophila melanogaster
  • Microtubule-Associated Proteins
  • Microtubules

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