Clathrin Heavy Chain Subunits Coordinate Endo- and Exocytic Traffic and Affect Stomatal Movement

Emily R Larson, Eva Van Zelm, Camille Roux, Annie Marion-Poll, Michael R Blatt

Research output: Contribution to journalArticle (Academic Journal)peer-review

Abstract

The current model for vesicular traffic to and from the plasma membrane is accepted, but the molecular requirements for this coordination are not well defined. We have identified the hot ABA-deficiency suppressor1 mutant, which has a stomatal function defect, as a clathrin heavy chain1 (CHC1) mutant allele and show that it has a decreased rate of endocytosis and growth defects that are shared with other chc1 mutant alleles. We used chc1 alleles and the related chc2 mutant as tools to investigate the effects that clathrin defects have on secretion pathways and plant growth. We show that secretion and endocytosis at the plasma membrane are sensitive to CHC1 and CHC2 function in seedling roots and that chc mutants have physiological defects in stomatal function and plant growth that have not been described previously. These findings suggest that clathrin supports specific functions in multiple cell types. Stomata movement and gas exchange are altered in chc mutants, indicating that clathrin is important for stomatal regulation. The aberrant function of chc mutant stomata is consistent with the growth phenotypes observed under different water and light conditions, which also are similar to those of the secretory SNARE mutant, syp121 The syp121 and chc mutants have impaired endocytosis and exocytosis compared with the wild type, indicating a link between SYP121-dependent secretion and clathrin-dependent endocytosis at the plasma membrane. Our findings provide evidence that clathrin and SYP121 functions are important for the coordination of endocytosis and exocytosis and have an impact on stomatal function, gas exchange, and vegetative growth in Arabidopsis (Arabidopsis thaliana).

Original languageEnglish
Pages (from-to)708-720
Number of pages13
JournalPlant Physiology
Volume175
Issue number2
DOIs
Publication statusPublished - Oct 2017

Bibliographical note

© 2017 The author(s). All Rights Reserved.

Keywords

  • Arabidopsis/cytology
  • Arabidopsis Proteins/genetics
  • Biological Transport
  • Cell Membrane/metabolism
  • Clathrin Heavy Chains/genetics
  • Endocytosis/genetics
  • Genotype
  • Plant Roots/cytology
  • Plant Stomata/cytology
  • Qa-SNARE Proteins/genetics
  • Seedlings/cytology

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