Cloning and characterization of a cDNA encoding rat PKR, the double-stranded RNA-dependent eukaryotic initiation factor-2 kinase

H Mellor, K M Flowers, S R Kimball, L S Jefferson

Research output: Contribution to journalArticle (Academic Journal)peer-review

Abstract

We have isolated and sequenced a full-length cDNA encoding the double-stranded RNA-dependent protein kinase PKR from rat. The derived amino acid sequences of the protein kinase and RNA-binding domains are highly conserved between the rat, human and mouse enzymes. In addition, sequence elements in the 5'- and 3'-untranslated regions are also conserved between species.

Original languageEnglish
Pages (from-to)693-6
Number of pages4
JournalBiochimica et Biophysica Acta (BBA)
Volume1219
Issue number3
DOIs
Publication statusPublished - 22 Nov 1994

Keywords

  • Animals
  • Base Sequence
  • Cloning, Molecular
  • Conserved Sequence
  • DNA, Complementary/genetics
  • Molecular Sequence Data
  • Protein-Serine-Threonine Kinases/genetics
  • RNA, Double-Stranded/metabolism
  • Rats
  • Sequence Homology, Nucleic Acid
  • Species Specificity
  • eIF-2 Kinase

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