Cloning and characterization of a cDNA encoding rat PKR, the double-stranded RNA-dependent eukaryotic initiation factor-2 kinase

H Mellor; K M Flowers; S R Kimball; L S Jefferson

doi:10.1016/0167-4781(94)90229-1

Cloning and characterization of a cDNA encoding rat PKR, the double-stranded RNA-dependent eukaryotic initiation factor-2 kinase

H Mellor, K M Flowers, S R Kimball, L S Jefferson

Research output: Contribution to journal › Article (Academic Journal) › peer-review

24 Citations (Scopus)

Abstract

We have isolated and sequenced a full-length cDNA encoding the double-stranded RNA-dependent protein kinase PKR from rat. The derived amino acid sequences of the protein kinase and RNA-binding domains are highly conserved between the rat, human and mouse enzymes. In addition, sequence elements in the 5'- and 3'-untranslated regions are also conserved between species.

Original language	English
Pages (from-to)	693-6
Number of pages	4
Journal	Biochimica et Biophysica Acta (BBA)
Volume	1219
Issue number	3
DOIs	https://doi.org/10.1016/0167-4781(94)90229-1
Publication status	Published - 22 Nov 1994

Keywords

Animals
Base Sequence
Cloning, Molecular
Conserved Sequence
DNA, Complementary/genetics
Molecular Sequence Data
Protein-Serine-Threonine Kinases/genetics
RNA, Double-Stranded/metabolism
Rats
Sequence Homology, Nucleic Acid
Species Specificity
eIF-2 Kinase

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title = "Cloning and characterization of a cDNA encoding rat PKR, the double-stranded RNA-dependent eukaryotic initiation factor-2 kinase",

abstract = "We have isolated and sequenced a full-length cDNA encoding the double-stranded RNA-dependent protein kinase PKR from rat. The derived amino acid sequences of the protein kinase and RNA-binding domains are highly conserved between the rat, human and mouse enzymes. In addition, sequence elements in the 5'- and 3'-untranslated regions are also conserved between species.",

keywords = "Animals, Base Sequence, Cloning, Molecular, Conserved Sequence, DNA, Complementary/genetics, Molecular Sequence Data, Protein-Serine-Threonine Kinases/genetics, RNA, Double-Stranded/metabolism, Rats, Sequence Homology, Nucleic Acid, Species Specificity, eIF-2 Kinase",

author = "H Mellor and Flowers, {K M} and Kimball, {S R} and Jefferson, {L S}",

year = "1994",

month = nov,

day = "22",

doi = "10.1016/0167-4781(94)90229-1",

language = "English",

volume = "1219",

pages = "693--6",

journal = "Biochimica et Biophysica Acta (BBA)",

issn = "0006-3002",

publisher = "Elsevier B.V.",

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TY - JOUR

T1 - Cloning and characterization of a cDNA encoding rat PKR, the double-stranded RNA-dependent eukaryotic initiation factor-2 kinase

AU - Mellor, H

AU - Flowers, K M

AU - Kimball, S R

AU - Jefferson, L S

PY - 1994/11/22

Y1 - 1994/11/22

N2 - We have isolated and sequenced a full-length cDNA encoding the double-stranded RNA-dependent protein kinase PKR from rat. The derived amino acid sequences of the protein kinase and RNA-binding domains are highly conserved between the rat, human and mouse enzymes. In addition, sequence elements in the 5'- and 3'-untranslated regions are also conserved between species.

AB - We have isolated and sequenced a full-length cDNA encoding the double-stranded RNA-dependent protein kinase PKR from rat. The derived amino acid sequences of the protein kinase and RNA-binding domains are highly conserved between the rat, human and mouse enzymes. In addition, sequence elements in the 5'- and 3'-untranslated regions are also conserved between species.

KW - Animals

KW - Base Sequence

KW - Cloning, Molecular

KW - Conserved Sequence

KW - DNA, Complementary/genetics

KW - Molecular Sequence Data

KW - Protein-Serine-Threonine Kinases/genetics

KW - RNA, Double-Stranded/metabolism

KW - Rats

KW - Sequence Homology, Nucleic Acid

KW - Species Specificity

KW - eIF-2 Kinase

U2 - 10.1016/0167-4781(94)90229-1

DO - 10.1016/0167-4781(94)90229-1

M3 - Article (Academic Journal)

C2 - 7948027

SN - 0006-3002

VL - 1219

SP - 693

EP - 696

JO - Biochimica et Biophysica Acta (BBA)

JF - Biochimica et Biophysica Acta (BBA)

IS - 3

ER -

Cloning and characterization of a cDNA encoding rat PKR, the double-stranded RNA-dependent eukaryotic initiation factor-2 kinase

Abstract

Keywords

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