Cloning and expression of a leech complexin

Iain M Dykes, Jane A Davies

Research output: Contribution to journalArticle (Academic Journal)peer-review

1 Citation (Scopus)

Abstract

Release of neurotransmitter from presynaptic nerve terminals is mediated by SNARE proteins, which are located on the vesicle and plasma membranes. These proteins form a SNARE complex thought to mediate membrane fusion. Complexin is a soluble protein essential for transmitter release, which has been postulated to bind to and stabilise the SNARE complex. We have cloned a complexin homologue, Hm-cpx1, from the leech, Hirudo medicinalis. This protein is expressed in only a subset of neurons in the leech CNS, including the Retzius and P neurons. It is 33% identical to rat complexin I, and 44% identical to squid complexin. Sequence conservation is particularly high in the predicted SNARE binding domain.

Original languageEnglish
Pages (from-to)93-7
Number of pages5
JournalGene Expression Patterns
Volume4
Issue number1
Publication statusPublished - Jan 2004

Keywords

  • Amino Acid Sequence
  • Animals
  • Central Nervous System
  • Cloning, Molecular
  • Gene Expression Profiling
  • In Situ Hybridization
  • Leeches
  • Molecular Sequence Data
  • Nerve Tissue Proteins
  • Phylogeny
  • Sequence Homology, Amino Acid

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