Abstract
Two full-length cDNA clones encoding alpha- and beta-subunits of a GABAA receptor have been isolated from a rat cerebral cortex cDNA library. The mature alpha-subunit protein consists of 428 amino acids with a calculated Mr of 48,680. This protein is highly homologous (approximately 99% amino acid identity) with the bovine brain alpha 1-subunit receptor [(1988) Nature 335, 76-79]. The mature rat beta-subunit receptor is a 448 amino acid polypeptide and shares approximately 80% amino acid identity with the previously characterized bovine GABAA receptor beta-subunit [(1987) Nature 328, 221-227]. Co-expression of the cloned DNA in Xenopus oocytes produces a functional receptor and ion channel with pharmacological characteristics of a GABAA receptor. GABAA alpha- and beta-subunit mRNA is detectable in the cortex, cerebellum and hippocampus.
| Original language | English |
|---|---|
| Pages (from-to) | 145-8 |
| Number of pages | 4 |
| Journal | FEBS Letters |
| Volume | 246 |
| Issue number | 1-2 |
| Publication status | Published - 27 Mar 1989 |
Keywords
- Amino Acid Sequence
- Animals
- Cattle
- Cerebellum
- Cerebral Cortex
- Cloning, Molecular
- DNA
- Gene Expression Regulation
- Glycosylation
- Hippocampus
- Molecular Sequence Data
- Molecular Weight
- Nucleic Acid Hybridization
- Oocytes
- Protein Sorting Signals
- RNA, Messenger
- Rats
- Receptors, GABA-A
- Sequence Homology, Nucleic Acid
- Transfection
- Xenopus
- gamma-Aminobutyric Acid