Co-culture of intestinal epithelial and stromal cells in 3D collagen-based environments

M E Viney, A J Bullock, M J Day, S MacNeil

Research output: Contribution to journalArticle (Academic Journal)

16 Citations (Scopus)

Abstract

AIM: To investigate the co-culture of established intestinal epithelial cell lines and stromal cells in a series of collagen-based environments for production of tissue-engineered intestinal epithelium for in vitro investigations.

MATERIALS & METHODS: Intestinal epithelial cells were co-cultured with fibroblasts on a range of supporting collagen matrices including commercially available Promogran and on collagen-based gels.

RESULTS: Epithelial growth was achieved with one combination of vimentin-expressing stromal and cytokeratin-expressing intestinal epithelial cells grown on collagen gels supplemented with Matrigel, and held at an air-liquid interface.

CONCLUSIONS: Collagen-based gels can support the co-culture of intestinal epithelial and stromal cells resulting in the growth of an epithelium that has some morphological similarity to normal intestinal tissue.

Original languageEnglish
Pages (from-to)397-406
Number of pages10
JournalRegenerative Medicine
Volume4
Issue number3
DOIs
Publication statusPublished - May 2009

Keywords

  • Animals
  • Cell Line
  • Coculture Techniques
  • Collagen
  • Epithelial Cells
  • Humans
  • Immunohistochemistry
  • Intestinal Mucosa
  • Rats
  • Stromal Cells
  • Tissue Engineering

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