Coassociation between Group B Streptococcus and Candida albicans Promotes Interactions with Vaginal Epithelium

Grace R. Pidwill, Sara Rego, Howard F. Jenkinson, Richard J. Lamont, Angela H. Nobbs*

*Corresponding author for this work

Research output: Contribution to journalArticle (Academic Journal)peer-review

12 Citations (Scopus)
210 Downloads (Pure)

Abstract

Group B Streptococcus (GBS) is a leading cause of neonatal sepsis, pneumonia and meningitis worldwide. In the majority of cases, GBS is transmitted vertically from mother to neonate, making maternal vaginal colonisation a key risk factor for neonatal disease. The fungus Candida albicans is an opportunistic pathogen of the female genitourinary tract, and the causative agent of vaginal thrush. Carriage of C. albicans has been shown to be an independent risk factor for vaginal colonisation by GBS. However, the nature of interactions between these two microbes is poorly understood. This study provides evidence of a reciprocal, synergistic interplay between GBS and C. albicans that may serve to promote their co-colonisation of the vaginal mucosa. GBS strains NEM316 (serotype III) and 515 (Ia) are shown to physically interact with C. albicans, with bacteria exhibiting tropism for candidal hyphal filaments. This interaction enhances association levels of both microbes with vaginal epithelial cell line VK2/E6E7. The ability of GBS to co-associate with C. albicans is dependent upon expression of hyphal-specific adhesin Als3. In turn, expression of GBS antigen I/II family adhesins (Bsp polypeptides) facilitates this co-association and confers upon surrogate Lactococcus lactis the capacity to exhibit enhanced interactions with C. albicans on vaginal epithelium. As genitourinary tract colonisation is an essential first step in the pathogenesis of GBS and C. albicans, the co-association mechanism reported here may have important implications for risk of disease involving both of these pathogens.
Original languageEnglish
Article numbere00669-17
Number of pages18
JournalInfection and Immunity
Volume86
Issue number4
Early online date16 Jan 2018
DOIs
Publication statusPublished - Apr 2018

Keywords

  • Adhesins
  • Candida albicans
  • Cocolonization
  • Polymicrobial interactions
  • Streptococcus agalactiae
  • Vaginal epithelium

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