Comparative performance of culture using swabs transported in Amies medium and the Aptima Combo 2 nucleic acid amplification test in detection of Neisseria gonorrhoeae from genital and extra-genital sites: a retrospective study

Lindsey Harryman; Sara Scofield; John Macleod; David Carrington; O Martin Williams; Arnold Fernandes; Paddy Horner

doi:10.1136/sextrans-2011-050075

Comparative performance of culture using swabs transported in Amies medium and the Aptima Combo 2 nucleic acid amplification test in detection of Neisseria gonorrhoeae from genital and extra-genital sites: a retrospective study

Lindsey Harryman, Sara Scofield, John Macleod, David Carrington, O Martin Williams, Arnold Fernandes, Paddy Horner

Research output: Contribution to journal › Article (Academic Journal) › peer-review

24 Citations (Scopus)

Abstract

Nucleic acid amplification tests are being increasingly used for the routine diagnosis of Neisseria gonorrhoeae (GC), although culture remains essential for monitoring antimicrobial resistance. The authors investigated how symptoms and infection site influenced test sensitivity.

Original language	English
Pages (from-to)	27-31
Number of pages	5
Journal	Sexually Transmitted Infections
Volume	88
Issue number	1
DOIs	https://doi.org/10.1136/sextrans-2011-050075
Publication status	Published - Feb 2012

Keywords

Culture Media
Female
Gonorrhea
Humans
Male
Neisseria gonorrhoeae
Nucleic Acid Amplification Techniques
Pharyngeal Diseases
Rectal Diseases
Retrospective Studies
Sensitivity and Specificity
Sex Factors
Specimen Handling
Urethral Diseases
Vaginal Diseases
Vulvar Diseases

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Harryman, L., Scofield, S., Macleod, J., Carrington, D., Williams, O. M., Fernandes, A., & Horner, P. (2012). Comparative performance of culture using swabs transported in Amies medium and the Aptima Combo 2 nucleic acid amplification test in detection of Neisseria gonorrhoeae from genital and extra-genital sites: a retrospective study. Sexually Transmitted Infections, 88(1), 27-31. https://doi.org/10.1136/sextrans-2011-050075

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title = "Comparative performance of culture using swabs transported in Amies medium and the Aptima Combo 2 nucleic acid amplification test in detection of Neisseria gonorrhoeae from genital and extra-genital sites: a retrospective study",

abstract = "Nucleic acid amplification tests are being increasingly used for the routine diagnosis of Neisseria gonorrhoeae (GC), although culture remains essential for monitoring antimicrobial resistance. The authors investigated how symptoms and infection site influenced test sensitivity.",

keywords = "Culture Media, Female, Gonorrhea, Humans, Male, Neisseria gonorrhoeae, Nucleic Acid Amplification Techniques, Pharyngeal Diseases, Rectal Diseases, Retrospective Studies, Sensitivity and Specificity, Sex Factors, Specimen Handling, Urethral Diseases, Vaginal Diseases, Vulvar Diseases",

author = "Lindsey Harryman and Sara Scofield and John Macleod and David Carrington and Williams, {O Martin} and Arnold Fernandes and Paddy Horner",

year = "2012",

month = feb,

doi = "10.1136/sextrans-2011-050075",

language = "English",

volume = "88",

pages = "27--31",

journal = "Sexually Transmitted Infections",

issn = "1368-4973",

publisher = "BMJ Publishing Group",

number = "1",

}

Harryman, L, Scofield, S, Macleod, J, Carrington, D, Williams, OM, Fernandes, A & Horner, P 2012, 'Comparative performance of culture using swabs transported in Amies medium and the Aptima Combo 2 nucleic acid amplification test in detection of Neisseria gonorrhoeae from genital and extra-genital sites: a retrospective study', Sexually Transmitted Infections, vol. 88, no. 1, pp. 27-31. https://doi.org/10.1136/sextrans-2011-050075

Comparative performance of culture using swabs transported in Amies medium and the Aptima Combo 2 nucleic acid amplification test in detection of Neisseria gonorrhoeae from genital and extra-genital sites: a retrospective study. / Harryman, Lindsey; Scofield, Sara; Macleod, John et al.
In: Sexually Transmitted Infections, Vol. 88, No. 1, 02.2012, p. 27-31.

Research output: Contribution to journal › Article (Academic Journal) › peer-review

TY - JOUR

T1 - Comparative performance of culture using swabs transported in Amies medium and the Aptima Combo 2 nucleic acid amplification test in detection of Neisseria gonorrhoeae from genital and extra-genital sites

T2 - a retrospective study

AU - Harryman, Lindsey

AU - Scofield, Sara

AU - Macleod, John

AU - Carrington, David

AU - Williams, O Martin

AU - Fernandes, Arnold

AU - Horner, Paddy

PY - 2012/2

Y1 - 2012/2

N2 - Nucleic acid amplification tests are being increasingly used for the routine diagnosis of Neisseria gonorrhoeae (GC), although culture remains essential for monitoring antimicrobial resistance. The authors investigated how symptoms and infection site influenced test sensitivity.

AB - Nucleic acid amplification tests are being increasingly used for the routine diagnosis of Neisseria gonorrhoeae (GC), although culture remains essential for monitoring antimicrobial resistance. The authors investigated how symptoms and infection site influenced test sensitivity.

KW - Culture Media

KW - Female

KW - Gonorrhea

KW - Humans

KW - Male

KW - Neisseria gonorrhoeae

KW - Nucleic Acid Amplification Techniques

KW - Pharyngeal Diseases

KW - Rectal Diseases

KW - Retrospective Studies

KW - Sensitivity and Specificity

KW - Sex Factors

KW - Specimen Handling

KW - Urethral Diseases

KW - Vaginal Diseases

KW - Vulvar Diseases

U2 - 10.1136/sextrans-2011-050075

DO - 10.1136/sextrans-2011-050075

M3 - Article (Academic Journal)

C2 - 22034496

SN - 1368-4973

VL - 88

SP - 27

EP - 31

JO - Sexually Transmitted Infections

JF - Sexually Transmitted Infections

IS - 1

ER -

Harryman L, Scofield S, Macleod J, Carrington D, Williams OM, Fernandes A et al. Comparative performance of culture using swabs transported in Amies medium and the Aptima Combo 2 nucleic acid amplification test in detection of Neisseria gonorrhoeae from genital and extra-genital sites: a retrospective study. Sexually Transmitted Infections. 2012 Feb;88(1):27-31. doi: 10.1136/sextrans-2011-050075