Comparative proteomics profiling reveals role of smooth muscle progenitors in extracellular matrix production

D Simper, U Mayr, C Urbich, A Zampetaki, M Prokopi, A Didangelos, A Saje, M Mueller, U Buehler, AC Newby, R Apweiler, S Rahman, S Dimmeler, Q Xu, M Mayr

Research output: Contribution to journalArticle (Academic Journal)peer-review

32 Citations (Scopus)

Abstract

OBJECTIVE: Recent studies on cardiovascular progenitors have led to a new appreciation that paracrine factors may support the regeneration of damaged tissues. METHODS AND RESULTS: We used a shotgun proteomics strategy to compare the secretome of peripheral blood-derived smooth muscle progenitors (SPCs) with human aortic smooth muscle cells. The late-outgrowth SPCs produced fewer proteolytic enzymes and inflammatory cytokines and showed reduced invasive capacity. Similar to smooth muscle cells, SPCs secreted extracellular matrix. However, SPCs produced different matrix proteins, as evidenced by the truncation of proangiogenic domains in collagen alpha-1 (I) and increased production of periostin. Moreover, SPCs retained serum proteins, including proteoglycans, regulating collagen assembly; and pigment epithelium-derived factor, a potent inhibitor of angiogenesis. As a functional consequence, their conditioned medium was less angiogenic, as demonstrated by endothelial tube formation assays in vitro and implantation of Matrigel plugs into nude, severe combined immunodeficient mice (NOD/SCID). CONCLUSIONS: The present study represents an important conceptual development, suggesting that SPCs may contribute to extracellular matrix production.
Translated title of the contributionComparative proteomics profiling reveals role of smooth muscle progenitors in extracellular matrix production
Original languageEnglish
Pages (from-to)1325 - 1332
Number of pages8
JournalArteriosclerosis, Thrombosis, and Vascular Biology
Volume30(7)
DOIs
Publication statusPublished - Jul 2010

Bibliographical note

Publisher: Arterioscler Thromb Vasc Biol

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