Comparative study of enterostatin sequence in five rat strains and enterostatin binding proteins in rat and chicken serum

Yingjen Jeffrey Wu, David Hughes, Ling Lin, Doug H Braymer, David A York

Research output: Contribution to journalArticle (Academic Journal)

13 Citations (Scopus)

Abstract

Enterostatin, a pentapeptide derived from the precursor protein procolipase has been shown to inhibit dietary fat intake and to reduce body fat after chronic administration in rats. We repeat that the enterostatin amino acid sequence from the genomic DNA of 5 different rat strains is APGPR. 125I-APGPR bound to three proteins (300, 205 and 60 kDa) in rat serum and one 60 kDa protein in chicken serum. These serum binding proteins were also eluted by APGPR affinity chromatography. Western blot analysis of serum protein identified enterostatin-like immunoreactivity associated with the same molecular weight bands. Our results demonstrate the enterostatin sequence in rat is APGPR and suggest the presence of enterostatin binding proteins in rat and chicken serum.

Original languageEnglish
Pages (from-to)537-44
Number of pages8
JournalPeptides
Volume23
Issue number3
Publication statusPublished - Mar 2002

Keywords

  • Animals
  • Blood Proteins/metabolism
  • Chickens
  • Chromatography, Affinity
  • Colipases/genetics
  • DNA/analysis
  • Enzyme Precursors
  • Genome
  • Iodine Radioisotopes
  • Male
  • Protein Precursors/genetics
  • Rats
  • Rats, Sprague-Dawley
  • Rats, Wistar
  • Rats, Zucker

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