Comparison of heterosubtypic protection in ferrets and pigs induced by a single-cycle influenza vaccine

Barbara Holzer, Sophie Morgan, Yumi Matsuoka, Matthew Edmans, Francisco Salguero, Helen Everett, Sharon Brookes, Emily Porter, Ronan MacLoughlin, Bryan Charleston, Kanta Subbarao, Alain Townsend, Elma Tchilian*

*Corresponding author for this work

Research output: Contribution to journalArticle (Academic Journal)peer-review

20 Citations (Scopus)
283 Downloads (Pure)

Abstract

Influenza is a major health threat, and a broadly protective influenza vaccine would be a significant advance. Signal Minus FLU (S-FLU) is a candidate broadly protective influenza vaccine that is limited to a single cycle of replication, which induces a strong cross-reactive T cell response but a minimal Ab response to hemagglutinin after intranasal or aerosol administration. We tested whether an H3N2 S-FLU can protect pigs and ferrets from heterosubtypic H1N1 influenza challenge. Aerosol administration of S-FLU to pigs induced lung tissue-resident memory T cells and reduced lung pathology but not the viral load. In contrast, in ferrets, S-FLU reduced viral replication and aerosol transmission. Our data show that S-FLU has different protective efficacy in pigs and ferrets, and that in the absence of Ab, lung T cell immunity can reduce disease severity without reducing challenge viral replication.

Original languageEnglish
Pages (from-to)4068-4077
Number of pages10
JournalJournal of Immunology
Volume200
Issue number12
Early online date4 Jun 2018
DOIs
Publication statusPublished - 15 Jun 2018

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