Abstract
Teeth are a model system in evolutionary and developmental biology, yet the evolutionary origins of this model organogenic system are poorly known. The principal laboratory models for dental research are Zebrafish (D anio rerio) and Mouse (M us musculus) . Both are highly derived organisms that diverged more than 400 million years ago. To better constrain ancestral patterns of tooth development and replacement, we employed Synchrotron Radiation Xray
Tomographic Microscopy at the TOMCAT beamline of the Swiss Light Source to characterise the the development of the dentition in the teleost actinopterygian Danio rerio and the non-teleost actinopterygian Polypterus senegalus. The resulting data were analysed and segmented digitally using computed tomography, allowing us to test established models of tooth development in
Danio and to compare these to the pattern of dental development in Polypterus and to sarcopterygian osteichthyans such as Mus. Our results show development and replacement of the dentition in Danio rerio, restricted to one pair of pharyngeal bones, and Polypterus, which possesses both pharyngeal and oral dentition. Both are compared to Mus, which shows a reduced oral dentition in the adult as compared to the embryo. Moreover, our high resolution, three-dimensional analysis provides additional insight into the spatial relations of developing dentitions, thus contributing complementary information to
previous studies.
Tomographic Microscopy at the TOMCAT beamline of the Swiss Light Source to characterise the the development of the dentition in the teleost actinopterygian Danio rerio and the non-teleost actinopterygian Polypterus senegalus. The resulting data were analysed and segmented digitally using computed tomography, allowing us to test established models of tooth development in
Danio and to compare these to the pattern of dental development in Polypterus and to sarcopterygian osteichthyans such as Mus. Our results show development and replacement of the dentition in Danio rerio, restricted to one pair of pharyngeal bones, and Polypterus, which possesses both pharyngeal and oral dentition. Both are compared to Mus, which shows a reduced oral dentition in the adult as compared to the embryo. Moreover, our high resolution, three-dimensional analysis provides additional insight into the spatial relations of developing dentitions, thus contributing complementary information to
previous studies.
| Original language | English |
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| Publication status | Published - 2015 |
| Event | Interdisciplinary Approaches in Fish Skeletal Biology - Tavira, Portugal Duration: 27 Apr 2015 → 30 Dec 2015 |
Conference
| Conference | Interdisciplinary Approaches in Fish Skeletal Biology |
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| Country/Territory | Portugal |
| City | Tavira |
| Period | 27/04/15 → 30/12/15 |