TY - JOUR
T1 - Compound-specific δ
15
N values express differences in amino acid metabolism in plants of varying lignin content
AU - Kendall, Iain P.
AU - Woodward, Peter
AU - Clark, Joshua P.
AU - Styring, Amy K.
AU - Hanna, John V.
AU - Evershed, Richard P.
PY - 2019/5
Y1 - 2019/5
N2 -
Amino acid δ
15
N values of foliage of various plant taxa, grown at the experimental farm stations of North Wyke, UK and Bad Lauchstädt, Germany were determined by GC-C-IRMS. The difference between δ
15
N values of glutamate (Glx) and phenylalanine (Phe) were found to differ significantly between woody and herbaceous plants, with mean Δ
15
N
Glx-Phe
(i.e. δ
15
N
Phe
- δ
15
N
Glx
) values of −9.3 ± 1.6‰ and −5.8 ± 2.1‰, respectively. These differences in values are hypothesised to be due to the involvement of Phe in the phenylpropanoid pathway, by which lignin and other phenolic secondary metabolites are produced, leading to isotopic fractionation and enrichment of the remaining Phe pool available for protein biosynthesis. This results in the more negative Δ
15
N
Glx-Phe
values observed in woody plants relative to herbaceous plants, as the former are assumed to produce more lignin. To test this assumption, plant leaf tissue lignin concentrations were estimated by solid state
13
C cross-polarisation, magic-angle-spinning (CPMAS) NMR spectroscopy for a subset of plants, which showed that tree foliage has a higher concentration of lignin (12.6 wt%) than herbaceous foliage (6.3 wt%). The correlation of lignin concentration with Δ
15
N
Glx-Phe
values demonstrates that the difference in these values with plant type is indeed due to differential production of lignin. The ability to estimate the lignin content of plants from amino acid δ
15
N values will, to give one example, allow refinement of estimates of herbivore diet in present and past ecosystems, enabling more accurate environmental niche modelling.
AB -
Amino acid δ
15
N values of foliage of various plant taxa, grown at the experimental farm stations of North Wyke, UK and Bad Lauchstädt, Germany were determined by GC-C-IRMS. The difference between δ
15
N values of glutamate (Glx) and phenylalanine (Phe) were found to differ significantly between woody and herbaceous plants, with mean Δ
15
N
Glx-Phe
(i.e. δ
15
N
Phe
- δ
15
N
Glx
) values of −9.3 ± 1.6‰ and −5.8 ± 2.1‰, respectively. These differences in values are hypothesised to be due to the involvement of Phe in the phenylpropanoid pathway, by which lignin and other phenolic secondary metabolites are produced, leading to isotopic fractionation and enrichment of the remaining Phe pool available for protein biosynthesis. This results in the more negative Δ
15
N
Glx-Phe
values observed in woody plants relative to herbaceous plants, as the former are assumed to produce more lignin. To test this assumption, plant leaf tissue lignin concentrations were estimated by solid state
13
C cross-polarisation, magic-angle-spinning (CPMAS) NMR spectroscopy for a subset of plants, which showed that tree foliage has a higher concentration of lignin (12.6 wt%) than herbaceous foliage (6.3 wt%). The correlation of lignin concentration with Δ
15
N
Glx-Phe
values demonstrates that the difference in these values with plant type is indeed due to differential production of lignin. The ability to estimate the lignin content of plants from amino acid δ
15
N values will, to give one example, allow refinement of estimates of herbivore diet in present and past ecosystems, enabling more accurate environmental niche modelling.
KW - nitrogen isotopes
KW - phenylpropaniod pathway
KW - solid state NMR
KW - GC-C-IRMS
UR - http://www.scopus.com/inward/record.url?scp=85062707376&partnerID=8YFLogxK
U2 - 10.1016/j.phytochem.2019.01.012
DO - 10.1016/j.phytochem.2019.01.012
M3 - Article (Academic Journal)
C2 - 30826700
AN - SCOPUS:85062707376
VL - 161
SP - 130
EP - 138
JO - Phytochemistry
JF - Phytochemistry
SN - 0031-9422
ER -