Construction and characterization of kilobasepair densely labeled peptide-DNA

Suzana Kovacic, Laleh Samii, Guillaume Lamour, Hongbin Li, Heiner Linke, Elizabeth H C Bromley, Derek N. Woolfson, Paul M G Curmi, Nancy R. Forde*

*Corresponding author for this work

Research output: Contribution to journalArticle (Academic Journal)peer-review

13 Citations (Scopus)

Abstract

Directed assembly of biocompatible materials benefits from modular building blocks in which structural organization is independent of introduced functional modifications. For soft materials, such modifications have been limited. Here, long DNA is successfully functionalized with dense decoration by peptides. Following introduction of alkyne-modified nucleotides into kilobasepair DNA, measurements of persistence length show that DNA mechanics are unaltered by the dense incorporation of alkynes (∼1 alkyne/2 bp) and after click-chemistry attachment of a tunable density of peptides. Proteolytic cleavage of densely tethered peptides (∼1 peptide/3 bp) demonstrates addressability of the functional groups, showing that this accessible approach to creating hybrid structures can maintain orthogonality between backbone mechanics and overlaid function. The synthesis and characterization of these hybrid constructs establishes the groundwork for their implementation in future applications, such as building blocks in modular approaches to a range of problems in synthetic biology.

Original languageEnglish
Pages (from-to)4065-4072
Number of pages8
JournalBiomacromolecules
Volume15
Issue number11
DOIs
Publication statusPublished - 18 Sep 2014

Structured keywords

  • Bristol BioDesign Institute

Keywords

  • SYNTHETIC BIOLOGY

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