Continuous fluorescence-based measurement of redox-driven sodium ion translocation.

Valentin Muras, Björn Claussen , Manikandan Karuppasamy, Julia Steuber, Christiane Schaffitzel

Research output: Contribution to journalArticle (Academic Journal)peer-review

2 Citations (Scopus)

Abstract

Investigation of the mechanism of sodium ion pumping enzymes requires methods to follow the translocation of sodium ions by the purified and reconstituted proteins in vitro. Here, we describe a protocol that allows following the accumulation of Na(+) in proteoliposomes by the Na(+)-translocating NADH:quinone oxidoreductase (Na(+)-NQR) from Vibrio cholerae using the sodium-sensitive fluorophor sodium green. In the presence of a regenerative system for its substrate NADH, the Na(+)-NQR accumulates Na(+) in the proteoliposomes which is visible as a change in fluorescence.
Original languageEnglish
Pages (from-to)53-55
Number of pages3
JournalAnalytical Biochemistry
Volume459
DOIs
Publication statusPublished - 15 Aug 2014

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