Control of repeat-protein curvature by computational protein design

Keunwan Park, Betty W Shen, Fabio Parmeggiani, Po-Ssu Huang, Barry L Stoddard, David Baker

Research output: Contribution to journalArticle (Academic Journal)

55 Citations (Scopus)

Abstract

Shape complementarity is an important component of molecular recognition, and the ability to precisely adjust the shape of a binding scaffold to match a target of interest would greatly facilitate the creation of high-affinity protein reagents and therapeutics. Here we describe a general approach to control the shape of the binding surface on repeat-protein scaffolds and apply it to leucine-rich-repeat proteins. First, self-compatible building-block modules are designed that, when polymerized, generate surfaces with unique but constant curvatures. Second, a set of junction modules that connect the different building blocks are designed. Finally, new proteins with custom-designed shapes are generated by appropriately combining building-block and junction modules. Crystal structures of the designs illustrate the power of the approach in controlling repeat-protein curvature.

Original languageEnglish
Pages (from-to)167-174
Number of pages8
JournalNature Structural and Molecular Biology
Volume22
Issue number2
Early online date12 Jan 2015
DOIs
Publication statusPublished - Feb 2015

Keywords

  • Crystallography, X-Ray
  • Protein Conformation
  • Protein Engineering
  • Proteins

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