Controlled release of dextrin-conjugated growth factors to support growth and differentiation of neural stem cells

Elaine L. Ferguson*, Sameza Naseer, Lydia C. Powell, Joseph Hardwicke, Fraser I. Young, Bangfu Zhu, Qian Liu, Bing Song, David W. Thomas

*Corresponding author for this work

Research output: Contribution to journalArticle (Academic Journal)peer-review

3 Citations (Scopus)
212 Downloads (Pure)

Abstract

An essential aspect of stem cell in vitro culture and in vivo therapy is achieving sustained levels of growth factors to support stem cell survival and expansion, while maintaining their multipotency and differentiation potential. This study investigated the ability of dextrin (~74,000 g/mol; 27.8 mol% succinoylation) conjugated to epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF; or FGF-2) (3.9 and 6.7% w/w protein loading, respectively) to support the expansion and differentiation of stem cells in vitro via sustained, controllable growth factor release. Supplementation of mouse neural stem cells (mNSCs) with dextrin-growth factor conjugates led to greater and prolonged proliferation compared to unbound EGF/bFGF controls, with no detectable apoptosis after 7 days of treatment. Immunocytochemical detection of neural precursor (nestin) and differentiation (Olig2, MAP2, GFAP) markers verified that controlled release of dextrin-conjugated growth factors preserves stem cell properties of mNSCs for up to 7 days. These results show the potential of dextrin-growth factor conjugates for localized delivery of bioactive therapeutic agents to support stem cell expansion and differentiation, and as an adjunct to direct neuronal repair.

Original languageEnglish
Pages (from-to)69-78
Number of pages10
JournalStem Cell Research
Volume33
Early online date6 Oct 2018
DOIs
Publication statusPublished - 1 Dec 2018

Keywords

  • Biodegradable polymers
  • Controlled release
  • Growth factors
  • Neural stem cells
  • Polymer therapeutics

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