Correlative light electron microscopy using small gold nanoparticles as single probes

Iestyn Pope, Hugh R C Tanner, Francesco Masia, Lukas Payne, Kenton Arkill, Judith M Mantell, Wolfgang Langbein, Paola Borri, Paul Verkade

Research output: Contribution to journalArticle (Academic Journal)peer-review

10 Citations (Scopus)

Abstract

Correlative light-electron microscopy (CLEM) requires the availability of robust probes which are visible both in light and electron microscopy. Here we demonstrate a CLEM approach using small gold nanoparticles as a single probe. Individual gold nanoparticles bound to the epidermal growth factor protein were located with nanometric precision background-free in human cancer cells by light microscopy using resonant four-wave mixing (FWM), and were correlatively mapped with high accuracy to the corresponding transmission electron microscopy images. We used nanoparticles of 10 nm and 5 nm radius, and show a correlation accuracy below 60 nm over an area larger than 10 µm size, without the need for additional fiducial markers. Correlation accuracy was improved to below 40 nm by reducing systematic errors, while the localisation precision is below 10 nm. Polarisation-resolved FWM correlates with nanoparticle shapes, promising for multiplexing by shape recognition in future applications. Owing to the photostability of gold nanoparticles and the applicability of FWM microscopy to living cells, FWM-CLEM opens up a powerful alternative to fluorescence-based methods.

Original languageEnglish
Article number80
Number of pages14
JournalLight, science & applications
Volume12
Issue number1
DOIs
Publication statusPublished - 29 Mar 2023

Bibliographical note

Funding Information:
This work was funded by the UK EPSRC Research Council (Grants EP/I005072/1, EP/I016260/1, EP/L001470/1, and EP/M028313/1) and the UK BBSRC Research Council (Grants BBL014181/1, BB/M001969/1).

Publisher Copyright:
© 2023, The Author(s).

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