Countercurrent chromatographic isolation and purification of 11′-α-tocomonoenol from the vitamin E extract of palm oil

Marco Müller, Simon Hammann, Walter Vetter

Research output: Contribution to journalArticle (Academic Journal)peer-review

243 Downloads (Pure)

Abstract

A new vitamin E homologue, α-tocomonoenol was detected in palm oil, but was not isolated in large amounts and with high purity so far. Here we present an easy and fast method to isolate α-tocomonoenol from vitamin E rich nutrient capsules with countercurrent chromatography (CCC). With the solvent system n-hexane – benzotrifluoride– acetonitrile (10:3.5:6.5, v/v/v) about 30mg α-tocomonoenol with a purity of 75% could be enrichedfrom 1g crude sample. Column chromatography with 20% deactivated silica gel and n-hexane – ethyl acetate (95:5, v/v) was performed to gain 5.6mg α-tocomonoenol with a purity of 99.5% according to GC/MS. Structural verification by ⁠1H NMR spectroscopy verified that the double bond was located in 11′-position (11′-α-tocomonoenol). The trace impurity detected in the isolate was identified to be 12′-α-tocomonoenol, a compound previously detected in marine samples.
Original languageEnglish
Pages (from-to)327-332
Number of pages6
JournalFood Chemistry
Volume256
Early online date26 Feb 2018
DOIs
Publication statusPublished - 1 Aug 2018

Keywords

  • Countercurrent chromatography
  • Tocomonoenol
  • Vitamin E
  • Palm Oil
  • GC/MS
  • NMR spectroscopy

Fingerprint Dive into the research topics of 'Countercurrent chromatographic isolation and purification of 11′-α-tocomonoenol from the vitamin E extract of palm oil'. Together they form a unique fingerprint.

Cite this