Countering elevated CO2 induced Fe and Zn reduction in Arabidopsis seeds

Peng Sun; Jean Charles Isner; Aude Coupel-Ledru; Qi Zhang; Ashley J. Pridgeon; Yaqian He; Paloma K. Menguer; Anthony J. Miller; Dale Sanders; Steve P. Mcgrath; Fonthip Noothong; Yun Kuan Liang; Alistair M. Hetherington

doi:10.1111/nph.18290

Countering elevated CO₂ induced Fe and Zn reduction in Arabidopsis seeds

Peng Sun, Jean Charles Isner, Aude Coupel-Ledru, Qi Zhang, Ashley J. Pridgeon, Yaqian He, Paloma K. Menguer, Anthony J. Miller, Dale Sanders, Steve P. Mcgrath, Fonthip Noothong, Yun Kuan Liang^*, Alistair M. Hetherington

^*Corresponding author for this work

Research output: Contribution to journal › Article (Academic Journal) › peer-review

11 Citations (Scopus)

Abstract

Growth at increased concentrations of CO₂ induces a reduction in seed zinc (Zn) and iron (Fe). Using Arabidopsis thaliana, we investigated whether this could be mitigated by reducing the elevated CO₂-induced decrease in transpiration. We used an infrared imaging-based screen to isolate mutants in At1g08080 that encodes ALPHA CARBONIC ANHYDRASE 7 (ACA7). aca7 mutant alleles display wild-type (WT) responses to abscisic acid (ABA) and light but are compromised in their response to elevated CO₂. ACA7 is expressed in guard cells. When aca7 mutants are grown at 1000 ppm CO₂ they exhibit higher transpiration and higher seed Fe and Zn content than WT grown under the same conditions. Our data show that by increasing transpiration it is possible to partially mitigate the reduction in seed Fe and Zn content when Arabidopsis is grown at elevated CO₂.

Original language	English
Pages (from-to)	1796-1806
Number of pages	11
Journal	New Phytologist
Volume	235
Issue number	5
Early online date	30 May 2022
DOIs	https://doi.org/10.1111/nph.18290
Publication status	E-pub ahead of print - 30 May 2022

Bibliographical note

Funding Information:
This work was supported by grants to AMH from the UK Biotechnology and Biological Sciences Research Council (BBSRC), grant no. BB/N001168/1 and the Leverhulme Trust. Y-KL was supported by the National Natural Science Foundation of China under grant no. 31770282. AJP is grateful to the UK Biotechnology and Biological Sciences Research Council for a PhD studentship funded through the South West Biosciences Doctoral Training Partnership (training grant reference: BB/M009122/1). AC-L received an individual Marie Curie Fellowship (MSCA-IF H2020). The CAPES Foundation within the Ministry of Education in Brazil programme ‘Science without Borders’ supported PKM. DS and AJM were supported by grants BB/P004474/1, BB/J00PR9796 and BB/J000PR9799 from the BBSRC and the John Innes Foundation.

Funding Information:
. This research was funded by His Majesty Trust Fund, grant number SR/SCI/CHEM/19/01, and by the Research Council of Tehran University of Medical Sciences

Publisher Copyright:
© 2022 The Authors. New Phytologist © 2022 New Phytologist Foundation.

Keywords

alpha carbonic anhydrases
Arabidopsis thaliana
CO signalling pathway
stomatal function
Zn and Fe

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10.1111/nph.18290

https://repository.rothamsted.ac.uk/item/98930/countering-elevated-co2-induced-fe-and-zn-reduction-in-arabidopsis-seedsLicence: CC BY

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Cite this

@article{c5a300ab740d40828a2e8e4e091f7d4a,

title = "Countering elevated CO2 induced Fe and Zn reduction in Arabidopsis seeds",

abstract = "Growth at increased concentrations of CO2 induces a reduction in seed zinc (Zn) and iron (Fe). Using Arabidopsis thaliana, we investigated whether this could be mitigated by reducing the elevated CO2-induced decrease in transpiration. We used an infrared imaging-based screen to isolate mutants in At1g08080 that encodes ALPHA CARBONIC ANHYDRASE 7 (ACA7). aca7 mutant alleles display wild-type (WT) responses to abscisic acid (ABA) and light but are compromised in their response to elevated CO2. ACA7 is expressed in guard cells. When aca7 mutants are grown at 1000 ppm CO2 they exhibit higher transpiration and higher seed Fe and Zn content than WT grown under the same conditions. Our data show that by increasing transpiration it is possible to partially mitigate the reduction in seed Fe and Zn content when Arabidopsis is grown at elevated CO2.",

keywords = "alpha carbonic anhydrases, Arabidopsis thaliana, CO signalling pathway, stomatal function, Zn and Fe",

author = "Peng Sun and Isner, \{Jean Charles\} and Aude Coupel-Ledru and Qi Zhang and Pridgeon, \{Ashley J.\} and Yaqian He and Menguer, \{Paloma K.\} and Miller, \{Anthony J.\} and Dale Sanders and Mcgrath, \{Steve P.\} and Fonthip Noothong and Liang, \{Yun Kuan\} and Hetherington, \{Alistair M.\}",

note = "Funding Information: This work was supported by grants to AMH from the UK Biotechnology and Biological Sciences Research Council (BBSRC), grant no. BB/N001168/1 and the Leverhulme Trust. Y-KL was supported by the National Natural Science Foundation of China under grant no. 31770282. AJP is grateful to the UK Biotechnology and Biological Sciences Research Council for a PhD studentship funded through the South West Biosciences Doctoral Training Partnership (training grant reference: BB/M009122/1). AC-L received an individual Marie Curie Fellowship (MSCA-IF H2020). The CAPES Foundation within the Ministry of Education in Brazil programme {\textquoteleft}Science without Borders{\textquoteright} supported PKM. DS and AJM were supported by grants BB/P004474/1, BB/J00PR9796 and BB/J000PR9799 from the BBSRC and the John Innes Foundation. Funding Information: . This research was funded by His Majesty Trust Fund, grant number SR/SCI/CHEM/19/01, and by the Research Council of Tehran University of Medical Sciences Publisher Copyright: {\textcopyright} 2022 The Authors. New Phytologist {\textcopyright} 2022 New Phytologist Foundation.",

year = "2022",

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doi = "10.1111/nph.18290",

language = "English",

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T1 - Countering elevated CO2 induced Fe and Zn reduction in Arabidopsis seeds

AU - Sun, Peng

AU - Isner, Jean Charles

AU - Coupel-Ledru, Aude

AU - Zhang, Qi

AU - Pridgeon, Ashley J.

AU - He, Yaqian

AU - Menguer, Paloma K.

AU - Miller, Anthony J.

AU - Sanders, Dale

AU - Mcgrath, Steve P.

AU - Noothong, Fonthip

AU - Liang, Yun Kuan

AU - Hetherington, Alistair M.

N1 - Funding Information: This work was supported by grants to AMH from the UK Biotechnology and Biological Sciences Research Council (BBSRC), grant no. BB/N001168/1 and the Leverhulme Trust. Y-KL was supported by the National Natural Science Foundation of China under grant no. 31770282. AJP is grateful to the UK Biotechnology and Biological Sciences Research Council for a PhD studentship funded through the South West Biosciences Doctoral Training Partnership (training grant reference: BB/M009122/1). AC-L received an individual Marie Curie Fellowship (MSCA-IF H2020). The CAPES Foundation within the Ministry of Education in Brazil programme ‘Science without Borders’ supported PKM. DS and AJM were supported by grants BB/P004474/1, BB/J00PR9796 and BB/J000PR9799 from the BBSRC and the John Innes Foundation. Funding Information: . This research was funded by His Majesty Trust Fund, grant number SR/SCI/CHEM/19/01, and by the Research Council of Tehran University of Medical Sciences Publisher Copyright: © 2022 The Authors. New Phytologist © 2022 New Phytologist Foundation.

PY - 2022/5/30

Y1 - 2022/5/30

N2 - Growth at increased concentrations of CO2 induces a reduction in seed zinc (Zn) and iron (Fe). Using Arabidopsis thaliana, we investigated whether this could be mitigated by reducing the elevated CO2-induced decrease in transpiration. We used an infrared imaging-based screen to isolate mutants in At1g08080 that encodes ALPHA CARBONIC ANHYDRASE 7 (ACA7). aca7 mutant alleles display wild-type (WT) responses to abscisic acid (ABA) and light but are compromised in their response to elevated CO2. ACA7 is expressed in guard cells. When aca7 mutants are grown at 1000 ppm CO2 they exhibit higher transpiration and higher seed Fe and Zn content than WT grown under the same conditions. Our data show that by increasing transpiration it is possible to partially mitigate the reduction in seed Fe and Zn content when Arabidopsis is grown at elevated CO2.

AB - Growth at increased concentrations of CO2 induces a reduction in seed zinc (Zn) and iron (Fe). Using Arabidopsis thaliana, we investigated whether this could be mitigated by reducing the elevated CO2-induced decrease in transpiration. We used an infrared imaging-based screen to isolate mutants in At1g08080 that encodes ALPHA CARBONIC ANHYDRASE 7 (ACA7). aca7 mutant alleles display wild-type (WT) responses to abscisic acid (ABA) and light but are compromised in their response to elevated CO2. ACA7 is expressed in guard cells. When aca7 mutants are grown at 1000 ppm CO2 they exhibit higher transpiration and higher seed Fe and Zn content than WT grown under the same conditions. Our data show that by increasing transpiration it is possible to partially mitigate the reduction in seed Fe and Zn content when Arabidopsis is grown at elevated CO2.

KW - alpha carbonic anhydrases

KW - Arabidopsis thaliana

KW - CO signalling pathway

KW - stomatal function

KW - Zn and Fe

UR - https://www.scopus.com/pages/publications/85132589184

U2 - 10.1111/nph.18290

DO - 10.1111/nph.18290

M3 - Article (Academic Journal)

C2 - 35637611

AN - SCOPUS:85132589184

SN - 0028-646X

VL - 235

SP - 1796

EP - 1806

JO - New Phytologist

JF - New Phytologist

IS - 5

ER -

Countering elevated CO₂ induced Fe and Zn reduction in Arabidopsis seeds

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The control of specificity in guard cell ROS-based signalling

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Countering elevated CO2 induced Fe and Zn reduction in Arabidopsis seeds

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The control of specificity in guard cell ROS-based signalling

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Countering elevated CO₂ induced Fe and Zn reduction in Arabidopsis seeds