CpG-free plasmids confer reduced inflammation and sustained pulmonary gene expression

S.C Hyde, I.A Pringle, S Abdullah, A.E Lawton, L.A Davies, A Varathalingam, G Nunez-Alonso, A.M Green, R.P Bazzani, S.G Sumner-Jones, M Chan, H Li, N.S Yew, S.H Cheng, A.C Boyd, J.C Davies, U Griesenbach, D.J Porteous, D.N Sheppard, F.M MunkongeE.W Alton, D.R Gill

Research output: Contribution to journalArticle (Academic Journal)peer-review

225 Citations (Scopus)

Abstract

Pulmonary delivery of plasmid DNA (pDNA)/cationic liposome complexes is associated with an acute unmethylated CG dinucleotide (CpG)-mediated inflammatory response and brief duration of transgene expression. We demonstrate that retention of even a single CpG in pDNA is sufficient to elicit an inflammatory response, whereas CpG-free pDNA vectors do not. Using a CpG-free pDNA expression vector, we achieved sustained (Z56 d) in vivo transgene expression in the absence of lung inflammation.
Translated title of the contributionCpG-free plasmids confer reduced inflammation and sustained pulmonary gene expression
Original languageEnglish
Pages (from-to)549 - 551
Number of pages3
JournalNature Biotechnology
Volume26
Issue number5
DOIs
Publication statusPublished - May 2008

Bibliographical note

Publisher: Nature Publishing Group
Other: Supplemental Data

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