Pulmonary delivery of plasmid DNA (pDNA)/cationic liposome complexes is associated with an acute unmethylated CG dinucleotide (CpG)-mediated inflammatory response and brief duration of transgene expression. We demonstrate that retention of even a single CpG in pDNA is sufficient to elicit an inflammatory response, whereas CpG-free pDNA vectors do not. Using a CpG-free pDNA expression vector, we achieved sustained (Z56 d) in vivo transgene expression in the absence of lung inflammation.
|Translated title of the contribution||CpG-free plasmids confer reduced inflammation and sustained pulmonary gene expression|
|Pages (from-to)||549 - 551|
|Number of pages||3|
|Publication status||Published - May 2008|
Publisher: Nature Publishing Group
Other: Supplemental Data