CpG-free plasmids confer reduced inflammation and sustained pulmonary gene expression

S.C Hyde; I.A Pringle; S Abdullah; A.E Lawton; L.A Davies; A Varathalingam; G Nunez-Alonso; A.M Green; R.P Bazzani; S.G Sumner-Jones; M Chan; H Li; N.S Yew; S.H Cheng; A.C Boyd; J.C Davies; U Griesenbach; D.J Porteous; D.N Sheppard; F.M Munkonge; E.W Alton; D.R Gill

doi:10.1038/nbt1399

CpG-free plasmids confer reduced inflammation and sustained pulmonary gene expression

S.C Hyde
, I.A Pringle
, S Abdullah
, A.E Lawton
, L.A Davies
, A Varathalingam
, G Nunez-Alonso
, A.M Green
, R.P Bazzani
, S.G Sumner-Jones
, M Chan
, H Li
, N.S Yew
, S.H Cheng
, A.C Boyd
, J.C Davies
, U Griesenbach
, D.J Porteous
, D.N Sheppard
, F.M Munkonge

E.W Alton, D.R Gill

Dynamic Cell Biology

Research output: Contribution to journal › Article (Academic Journal) › peer-review

277 Citations (Scopus)

Abstract

Pulmonary delivery of plasmid DNA (pDNA)/cationic liposome complexes is associated with an acute unmethylated CG dinucleotide (CpG)-mediated inflammatory response and brief duration of transgene expression. We demonstrate that retention of even a single CpG in pDNA is sufficient to elicit an inflammatory response, whereas CpG-free pDNA vectors do not. Using a CpG-free pDNA expression vector, we achieved sustained (Z56 d) in vivo transgene expression in the absence of lung inflammation.

Translated title of the contribution	CpG-free plasmids confer reduced inflammation and sustained pulmonary gene expression
Original language	English
Pages (from-to)	549 - 551
Number of pages	3
Journal	Nature Biotechnology
Volume	26
Issue number	5
DOIs	https://doi.org/10.1038/nbt1399
Publication status	Published - May 2008

Bibliographical note

Publisher: Nature Publishing Group
Other: Supplemental Data

Access to Document

10.1038/nbt1399

http://www.nature.com/nbt/journal/v26/n5/full/nbt1399.html

Handle.net

Persistent link

Cite this

Hyde, S. C., Pringle, I. A., Abdullah, S., Lawton, A. E., Davies, L. A., Varathalingam, A., Nunez-Alonso, G., Green, A. M., Bazzani, R. P., Sumner-Jones, S. G., Chan, M., Li, H., Yew, N. S., Cheng, S. H., Boyd, A. C., Davies, J. C., Griesenbach, U., Porteous, D. J., Sheppard, D. N., ... Gill, D. R. (2008). CpG-free plasmids confer reduced inflammation and sustained pulmonary gene expression. Nature Biotechnology, 26(5), 549 - 551. https://doi.org/10.1038/nbt1399

@article{12a7b79b1f2c475eacd04e9e9a2f828f,

title = "CpG-free plasmids confer reduced inflammation and sustained pulmonary gene expression",

abstract = "Pulmonary delivery of plasmid DNA (pDNA)/cationic liposome complexes is associated with an acute unmethylated CG dinucleotide (CpG)-mediated inflammatory response and brief duration of transgene expression. We demonstrate that retention of even a single CpG in pDNA is sufficient to elicit an inflammatory response, whereas CpG-free pDNA vectors do not. Using a CpG-free pDNA expression vector, we achieved sustained (Z56 d) in vivo transgene expression in the absence of lung inflammation.",

author = "S.C Hyde and I.A Pringle and S Abdullah and A.E Lawton and L.A Davies and A Varathalingam and G Nunez-Alonso and A.M Green and R.P Bazzani and S.G Sumner-Jones and M Chan and H Li and N.S Yew and S.H Cheng and A.C Boyd and J.C Davies and U Griesenbach and D.J Porteous and D.N Sheppard and F.M Munkonge and E.W Alton and D.R Gill",

note = "Publisher: Nature Publishing Group Other: Supplemental Data",

year = "2008",

month = may,

doi = "10.1038/nbt1399",

language = "English",

volume = "26",

pages = "549 -- 551",

journal = "Nature Biotechnology",

issn = "1087-0156",

publisher = "Nature Research",

number = "5",

}

Hyde, SC, Pringle, IA, Abdullah, S, Lawton, AE, Davies, LA, Varathalingam, A, Nunez-Alonso, G, Green, AM, Bazzani, RP, Sumner-Jones, SG, Chan, M, Li, H, Yew, NS, Cheng, SH, Boyd, AC, Davies, JC, Griesenbach, U, Porteous, DJ, Sheppard, DN, Munkonge, FM, Alton, EW & Gill, DR 2008, 'CpG-free plasmids confer reduced inflammation and sustained pulmonary gene expression', Nature Biotechnology, vol. 26, no. 5, pp. 549 - 551. https://doi.org/10.1038/nbt1399

TY - JOUR

T1 - CpG-free plasmids confer reduced inflammation and sustained pulmonary gene expression

AU - Hyde, S.C

AU - Pringle, I.A

AU - Abdullah, S

AU - Lawton, A.E

AU - Davies, L.A

AU - Varathalingam, A

AU - Nunez-Alonso, G

AU - Green, A.M

AU - Bazzani, R.P

AU - Sumner-Jones, S.G

AU - Chan, M

AU - Li, H

AU - Yew, N.S

AU - Cheng, S.H

AU - Boyd, A.C

AU - Davies, J.C

AU - Griesenbach, U

AU - Porteous, D.J

AU - Sheppard, D.N

AU - Munkonge, F.M

AU - Alton, E.W

AU - Gill, D.R

N1 - Publisher: Nature Publishing Group Other: Supplemental Data

PY - 2008/5

Y1 - 2008/5

N2 - Pulmonary delivery of plasmid DNA (pDNA)/cationic liposome complexes is associated with an acute unmethylated CG dinucleotide (CpG)-mediated inflammatory response and brief duration of transgene expression. We demonstrate that retention of even a single CpG in pDNA is sufficient to elicit an inflammatory response, whereas CpG-free pDNA vectors do not. Using a CpG-free pDNA expression vector, we achieved sustained (Z56 d) in vivo transgene expression in the absence of lung inflammation.

AB - Pulmonary delivery of plasmid DNA (pDNA)/cationic liposome complexes is associated with an acute unmethylated CG dinucleotide (CpG)-mediated inflammatory response and brief duration of transgene expression. We demonstrate that retention of even a single CpG in pDNA is sufficient to elicit an inflammatory response, whereas CpG-free pDNA vectors do not. Using a CpG-free pDNA expression vector, we achieved sustained (Z56 d) in vivo transgene expression in the absence of lung inflammation.

UR - https://www.scopus.com/pages/publications/43449111490

U2 - 10.1038/nbt1399

DO - 10.1038/nbt1399

M3 - Article (Academic Journal)

C2 - 18438402

SN - 1087-0156

VL - 26

SP - 549

EP - 551

JO - Nature Biotechnology

JF - Nature Biotechnology

IS - 5

ER -

CpG-free plasmids confer reduced inflammation and sustained pulmonary gene expression

Abstract

Bibliographical note

Access to Document

Handle.net

Other files and links

Fingerprint

Cite this