Cprp - An unusual, repetitive protein which impacts pleuromutilin biosynthesis in the basidiomycete Clitopilus passeckerianus

Kate M J De Mattos-Shipley*, Gary D Foster, Andy M Bailey*

*Corresponding author for this work

Research output: Contribution to journalArticle (Academic Journal)peer-review

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Interrogation of an EST database for Clitopilus passeckerianus identified a putative homologue to the unusual stress response gene from yeast; ddr48, as being upregulated under pleuromutilin production conditions. Silencing of this gene, named cprp, produced a population of transformants which demonstrated significantly reduced pleuromutilin production. Attempts to complement a Saccharomyces cerevisiae ddr48 mutant strain (strain Y16748) with cprp were hampered by the lack of a clearly identifiable mutant phenotype, but interestingly, overexpression of either ddr48 or cprp in S. cerevisiae Y16748 led to a conspicuous and comparable reduction in growth rate. This observation, combined with the known role of DDR48 proteins from a range of fungal species in nutrient starvation and stress responses, raises the possibility that this family of proteins plays a role in triggering oligotrophic growth. Localisation studies via the production of a Cprp:GFP fusion protein in C. passeckerianus showed clear localisation adjacent to the hyphal septa and, to a lesser extent, cell walls, which is consistent with the identification of DDR48 as a cell wall-associated protein in various yeast species. To our knowledge this is the first study demonstrating that a DDR48-like protein plays a role in the regulation of a secondary metabolite, and represents the first DDR48-like protein from a basidiomycete. Potential homologues can be identified across much of the Dikarya, suggesting that this unusual protein may play a central role in regulating both primary and secondary metabolism in fungi.
Original languageEnglish
Article number655323
Number of pages16
JournalFrontiers in Fungal Biology
Publication statusPublished - 6 Apr 2021


  • Pleuromutilin
  • secondary metabolism
  • CPRP
  • DDR48
  • Clitopilus passeckerianus
  • Basidiomycete
  • antibiotic


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