CreC Sensor Kinase Activation Enhances Growth of Escherichia coli in the Presence of Cephalosporins and Carbapenems

Yuiko Takebayashi; Emma S Taylor; Sam Whitwam; Matthew B Avison

doi:10.1128/AAC.00846-19

CreC Sensor Kinase Activation Enhances Growth of Escherichia coli in the Presence of Cephalosporins and Carbapenems

Yuiko Takebayashi
, Emma S Taylor
, Sam Whitwam
, Matthew B Avison

Research output: Contribution to journal › Article (Academic Journal) › peer-review

1 Citation (Scopus)

153 Downloads (Pure)

Abstract

Mutants with enhanced growth in the presence of an antibiotic are more difficult to identify than mutants where the antibiotic's minimum inhibitory concentration increases, because they are not amenable to lethal selection in vitro. We report that activatory mutations in the CreC signal sensor enhance growth of Escherichia coli in the presence of cefoxitin, cefotaxime and meropenem without increasing their minimum inhibitory concentrations. Enhanced growth is dependent on over-production of the inner-membrane cre-regulon protein CreD.

Original language	English
Article number	e00846-19
Number of pages	16
Journal	Antimicrobial Agents and Chemotherapy
Volume	63
Issue number	11
Early online date	26 Aug 2019
DOIs	https://doi.org/10.1128/AAC.00846-19
Publication status	Published - 1 Nov 2019

Keywords

Fitness
Tolerance
Two-component system

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10.1128/AAC.00846-19

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This is the author accepted manuscript (AAM). The final published version (version of record) is available online via American Society for Microbiology at https://aac.asm.org/content/early/2019/08/21/AAC.00846-19 . Please refer to any applicable terms of use of the publisher.
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abstract = "Mutants with enhanced growth in the presence of an antibiotic are more difficult to identify than mutants where the antibiotic's minimum inhibitory concentration increases, because they are not amenable to lethal selection in vitro. We report that activatory mutations in the CreC signal sensor enhance growth of Escherichia coli in the presence of cefoxitin, cefotaxime and meropenem without increasing their minimum inhibitory concentrations. Enhanced growth is dependent on over-production of the inner-membrane cre-regulon protein CreD.",

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AU - Avison, Matthew B

PY - 2019/11/1

Y1 - 2019/11/1

N2 - Mutants with enhanced growth in the presence of an antibiotic are more difficult to identify than mutants where the antibiotic's minimum inhibitory concentration increases, because they are not amenable to lethal selection in vitro. We report that activatory mutations in the CreC signal sensor enhance growth of Escherichia coli in the presence of cefoxitin, cefotaxime and meropenem without increasing their minimum inhibitory concentrations. Enhanced growth is dependent on over-production of the inner-membrane cre-regulon protein CreD.

AB - Mutants with enhanced growth in the presence of an antibiotic are more difficult to identify than mutants where the antibiotic's minimum inhibitory concentration increases, because they are not amenable to lethal selection in vitro. We report that activatory mutations in the CreC signal sensor enhance growth of Escherichia coli in the presence of cefoxitin, cefotaxime and meropenem without increasing their minimum inhibitory concentrations. Enhanced growth is dependent on over-production of the inner-membrane cre-regulon protein CreD.

KW - Fitness

KW - Tolerance

KW - Two-component system

UR - https://www.scopus.com/pages/publications/85073764400

U2 - 10.1128/AAC.00846-19

DO - 10.1128/AAC.00846-19

M3 - Article (Academic Journal)

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IS - 11

M1 - e00846-19

ER -

CreC Sensor Kinase Activation Enhances Growth of Escherichia coli in the Presence of Cephalosporins and Carbapenems

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