Crossbridge mechanism(s) examined by temperature perturbation studies on muscle

KW Ranatunga, ME Coupland

Research output: Contribution to journalArticle (Academic Journal)peer-review

4 Citations (Scopus)

Abstract

An overall view of the contractile process that has emerged from -temperature-studies on active muscle is outlined. In isometric muscle, a small rapid temperature-jump (T-jump) enhances an early, pre-phosphate release, step in the acto-myosin (crossbridge) ATPase cycle and induces a characteristic rise in force indicating that crossbridge force generation is endothermic (force rises when heat is absorbed). Sigmoidal temperature dependence of steady force is largely due to the endothermic nature of force generation. During shortening, when muscle force is decreased, the T-jump force generation is enhanced; conversely, when a muscle is lengthening and its force increased, the T-jump force generation is inhibited. Taking T-jump force generation as a signature of the crossbridge - ATPase cycle, the results suggest that during lengthening the ATPase cycle is truncated before endothermic force generation, whereas during shortening this step and the ATPase cycle, are accelerated; this readily provides a molecular basis for the Fenn effect.
Translated title of the contributionCrossbridge mechanism(s) examined by temperature perturbation studies on muscle
Original languageEnglish
Pages (from-to)247 - 266
Number of pages21
JournalAdvances in Experimental Medicine and Biology
Volume682
DOIs
Publication statusPublished - 2010

Bibliographical note

Publisher: Springer New York

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