Cryo-EM Structure of Saccharomyces cerevisiae Target of Rapamycin Complex 2

Manikandan Karuppasamy, Beata Kusmider, Taiana M. Oliveira, Christl Gaubitz, Manoel Prouteau, Robbie Loewith, Christiane Schaffitzel

Research output: Contribution to journalArticle (Academic Journal)peer-review

30 Citations (Scopus)
430 Downloads (Pure)

Abstract

The Target Of Rapamycin (TOR) kinase assembles into two, distinct multiprotein complexes, conserved across eukaryote evolution. In contrast to TOR Complex1 (TORC1), TORC2 kinase activity is not inhibited by the macrolide rapamycin. Here, we present the structure of Saccharomyces cerevisiae TORC2 determined by electron cryo-microscopy. TORC2 contains six subunits assembling into a 1.4 MDa rhombohedron. Tor2 and Lst8 form the common core of both TOR complexes. Avo3/Rictor is unique to TORC2, but interacts with the same HEAT repeats of Tor2 that are engaged by Kog1/Raptor in mammalian TORC1, explaining the mutual exclusivity of these two proteins. Density, which we conclude is Avo3, occludes the FKBP12-rapamycin-binding site of Tor2’s FRB-domain rendering TORC2 rapamycin-insensitive and recessing the kinase active-site. Although mobile, Avo1/hSin1 further restricts access to the active site as its Conserved-Region-In-the-Middle (CRIM) domain is positioned along an edge of the TORC2 active-site-cleft, consistent with a role for CRIM in substrate recruitment.
Original languageEnglish
Article number1729
Number of pages18
JournalNature Communications
Volume8
DOIs
Publication statusPublished - 23 Nov 2017

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