Cryo-EM structure of the E. coli translating ribosome in complex with SRP and its receptor

Leandro F Estrozi, Daniel Boehringer, Shu-Ou Shan, Nenad Ban, Christiane Schaffitzel

Research output: Contribution to journalArticle (Academic Journal)peer-review

58 Citations (Scopus)

Abstract

We report the 'early' conformation of the Escherichia coli signal recognition particle (SRP) and its receptor FtsY bound to the translating ribosome, as determined by cryo-EM. FtsY binds to the tetraloop of the SRP RNA, whereas the NG domains of the SRP protein and FtsY interact weakly in this conformation. Our results suggest that optimal positioning of the SRP RNA tetraloop and the Ffh NG domain leads to FtsY recruitment.

Original languageEnglish
Pages (from-to)88-90
Number of pages3
JournalNature Structural and Molecular Biology
Volume18
Issue number1
DOIs
Publication statusPublished - Jan 2011

Keywords

  • Bacterial Proteins
  • Cryoelectron Microscopy
  • Escherichia coli
  • Escherichia coli Proteins
  • Models, Molecular
  • Protein Biosynthesis
  • Protein Structure, Tertiary
  • Receptors, Cytoplasmic and Nuclear
  • Receptors, Peptide
  • Ribosomes
  • Signal Recognition Particle

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