Abstract
We report the 'early' conformation of the Escherichia coli signal recognition particle (SRP) and its receptor FtsY bound to the translating ribosome, as determined by cryo-EM. FtsY binds to the tetraloop of the SRP RNA, whereas the NG domains of the SRP protein and FtsY interact weakly in this conformation. Our results suggest that optimal positioning of the SRP RNA tetraloop and the Ffh NG domain leads to FtsY recruitment.
Original language | English |
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Pages (from-to) | 88-90 |
Number of pages | 3 |
Journal | Nature Structural and Molecular Biology |
Volume | 18 |
Issue number | 1 |
DOIs | |
Publication status | Published - Jan 2011 |
Keywords
- Bacterial Proteins
- Cryoelectron Microscopy
- Escherichia coli
- Escherichia coli Proteins
- Models, Molecular
- Protein Biosynthesis
- Protein Structure, Tertiary
- Receptors, Cytoplasmic and Nuclear
- Receptors, Peptide
- Ribosomes
- Signal Recognition Particle