Crystallization and preliminary X-ray analysis of the bacillaene synthase trans-acting acyltransferase PksC

F Cuskin, AS Solovyova, RJ Lewis, PR Race

Research output: Contribution to journalArticle (Academic Journal)peer-review

5 Citations (Scopus)

Abstract

The antibiotic bacillaene is biosynthesized in Bacillus subtilis by a hybrid type 1 modular polyketide synthase/nonribosomal peptide synthetase of the transacyltransferase (trans-AT) class. Within this system, the essential acyl-group loading activity is provided by the action of three free-standing trans-acting acyltransferases. Here, the recombinant expression, purification and crystallization of the bacillaene synthase trans-acting acyltransferase PksC are reported. A diffraction data set has been collected from a single PksC crystal to 1.44 A ° resolution and the crystal was found to belong to the orthorhombic space group P212121.
Translated title of the contributionCrystallization and preliminary X-ray analysis of the bacillaene synthase trans-acting acyltransferase PksC
Original languageEnglish
Pages (from-to)464 - 466
Number of pages3
JournalActa Crystallographica Section F: Structural Biology and Crystallization Communications
Volume67
Issue number4
DOIs
Publication statusPublished - Apr 2011

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