Crystallization of a soluble form of the rat T-cell surface glycoprotein CD4 complexed with Fab from the W3/25 monoclonal antibody

Simon J. Davis*, R. Leo Brady, A. Neil Barclay, Karl Harlos, G. Guy Dodson, Alan F. Williams

*Corresponding author for this work

Research output: Contribution to journalArticle (Academic Journal)

23 Citations (Scopus)

Abstract

The structure of the T-lymphocyte cell surface glycoprotein CD4 is of considerable biological and medical interest. Recombinant rat CD4 expressed in soluble form in mammalian cells and complexed with W3/25 monoclonal Fab fragments formed crystals that diffract to 3·5 Å and have the orthorhombic space group P21212 or P212121. The unit cell has dimensions a = 317 Å, b = 161 Å and c = 41·8 Å and the asymmetric unit consists of two CD4 : Fab complexes. These crystals are of suitable quality for X-ray diffraction analysis.

Original languageEnglish
Pages (from-to)7-10
Number of pages4
JournalJournal of Molecular Biology
Volume213
Issue number1
DOIs
Publication statusPublished - 5 May 1990

Fingerprint Dive into the research topics of 'Crystallization of a soluble form of the rat T-cell surface glycoprotein CD4 complexed with Fab from the W3/25 monoclonal antibody'. Together they form a unique fingerprint.

Cite this