Crystallization of a soluble form of the rat T-cell surface glycoprotein CD4 complexed with Fab from the W3/25 monoclonal antibody

Simon J. Davis; R. Leo Brady; A. Neil Barclay; Karl Harlos; G. Guy Dodson; Alan F. Williams

doi:10.1016/S0022-2836(05)80116-0

Crystallization of a soluble form of the rat T-cell surface glycoprotein CD4 complexed with Fab from the W3/25 monoclonal antibody

Simon J. Davis^*
, R. Leo Brady
, A. Neil Barclay
, Karl Harlos
, G. Guy Dodson
, Alan F. Williams

^*Corresponding author for this work

Research output: Contribution to journal › Article (Academic Journal) › peer-review

29 Citations (Scopus)

Abstract

The structure of the T-lymphocyte cell surface glycoprotein CD4 is of considerable biological and medical interest. Recombinant rat CD4 expressed in soluble form in mammalian cells and complexed with W3/25 monoclonal Fab fragments formed crystals that diffract to 3·5 Å and have the orthorhombic space group P2₁2₁2 or P2₁2₁2₁. The unit cell has dimensions a = 317 Å, b = 161 Å and c = 41·8 Å and the asymmetric unit consists of two CD4 : Fab complexes. These crystals are of suitable quality for X-ray diffraction analysis.

Original language	English
Pages (from-to)	7-10
Number of pages	4
Journal	Journal of Molecular Biology
Volume	213
Issue number	1
DOIs	https://doi.org/10.1016/S0022-2836(05)80116-0
Publication status	Published - 5 May 1990

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Inorganic & Materials

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@article{a92abf7f405c439ba0b6115eca658f76,

title = "Crystallization of a soluble form of the rat T-cell surface glycoprotein CD4 complexed with Fab from the W3/25 monoclonal antibody",

abstract = "The structure of the T-lymphocyte cell surface glycoprotein CD4 is of considerable biological and medical interest. Recombinant rat CD4 expressed in soluble form in mammalian cells and complexed with W3/25 monoclonal Fab fragments formed crystals that diffract to 3·5 {\AA} and have the orthorhombic space group P21212 or P212121. The unit cell has dimensions a = 317 {\AA}, b = 161 {\AA} and c = 41·8 {\AA} and the asymmetric unit consists of two CD4 : Fab complexes. These crystals are of suitable quality for X-ray diffraction analysis.",

author = "Davis, \{Simon J.\} and Brady, \{R. Leo\} and Barclay, \{A. Neil\} and Karl Harlos and Dodson, \{G. Guy\} and Williams, \{Alan F.\}",

year = "1990",

month = may,

day = "5",

doi = "10.1016/S0022-2836(05)80116-0",

language = "English",

volume = "213",

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journal = "Journal of Molecular Biology",

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T1 - Crystallization of a soluble form of the rat T-cell surface glycoprotein CD4 complexed with Fab from the W3/25 monoclonal antibody

AU - Davis, Simon J.

AU - Brady, R. Leo

AU - Barclay, A. Neil

AU - Harlos, Karl

AU - Dodson, G. Guy

AU - Williams, Alan F.

PY - 1990/5/5

Y1 - 1990/5/5

N2 - The structure of the T-lymphocyte cell surface glycoprotein CD4 is of considerable biological and medical interest. Recombinant rat CD4 expressed in soluble form in mammalian cells and complexed with W3/25 monoclonal Fab fragments formed crystals that diffract to 3·5 Å and have the orthorhombic space group P21212 or P212121. The unit cell has dimensions a = 317 Å, b = 161 Å and c = 41·8 Å and the asymmetric unit consists of two CD4 : Fab complexes. These crystals are of suitable quality for X-ray diffraction analysis.

AB - The structure of the T-lymphocyte cell surface glycoprotein CD4 is of considerable biological and medical interest. Recombinant rat CD4 expressed in soluble form in mammalian cells and complexed with W3/25 monoclonal Fab fragments formed crystals that diffract to 3·5 Å and have the orthorhombic space group P21212 or P212121. The unit cell has dimensions a = 317 Å, b = 161 Å and c = 41·8 Å and the asymmetric unit consists of two CD4 : Fab complexes. These crystals are of suitable quality for X-ray diffraction analysis.

UR - https://www.scopus.com/pages/publications/0025353362

U2 - 10.1016/S0022-2836(05)80116-0

DO - 10.1016/S0022-2836(05)80116-0

M3 - Article (Academic Journal)

C2 - 2338715

AN - SCOPUS:0025353362

SN - 0022-2836

VL - 213

SP - 7

EP - 10

JO - Journal of Molecular Biology

JF - Journal of Molecular Biology

IS - 1

ER -

Crystallization of a soluble form of the rat T-cell surface glycoprotein CD4 complexed with Fab from the W3/25 monoclonal antibody

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